Product Info Summary
SKU: | PB9217 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HOXA10 Antibody Picoband®
SKU/Catalog Number
PB9217
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HOXA10 Antibody Picoband® catalog # PB9217. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HOXA10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9217)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HOXA10 recombinant protein (Position: Y57-R340). Human HOXA10 shares 87% amino acid (aa) sequence identity with mouse HOXA10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9217 is reactive to HOXA10 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
42414 MW
Background of HOXA10
Homeobox protein Hox-A10 is a protein that in humans is encoded by the HOXA10 gene. HOXA10 is expressed in the adult human endometrium, and the expression of HOXA10 dramatically increased during the midsecretory phase of the menstrual cycle, corresponding to the time of implantation and increase in circulating progesterone. HOXA10 may have an important function in regulating endometrial development during the menstrual cycle and in establishing conditions necessary for implantation in the human. HOXA10 is also a master regulator of postnatal hematopoietic development whose tight regulation is required for normal development of erythroid and megakaryocytic lineages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9217 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Kidney Tissue, Human Placenta Tissue, HELA Whole Cell, SW620 Whole Cell, HEPA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-HOXA10 antibody, PB9217, Western blotting
All lanes: Anti HOXA10 (PB9217) at 0.5ug/ml
WB: Recombinant Human HOXA10 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Click image to see more details
Anti-HOXA10 antibody, PB9217, Western blotting
All lanes: Anti HOXA10 (PB9217) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
Protein Target Info & Infographic
Gene/Protein Information For HOXA10 (Source: Uniprot.org, NCBI)
Gene Name
HOXA10
Full Name
Homeobox protein Hox-A10
Weight
42414 MW
Superfamily
Abd-B homeobox family
Alternative Names
Homeobox protein Hox-A10;Homeobox protein Hox-1.8;Homeobox protein Hox-1H;PL;HOXA10;HOX1H; HOXA10 HOX1, HOX1.8, HOX1H, PL homeobox A10 homeobox protein Hox-A10|homeo box A10|homeobox protein 1H|homeobox protein HOXA10|homeobox protein Hox-1.8|homeobox protein Hox-1H
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOXA10, check out the HOXA10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOXA10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HOXA10 Antibody Picoband® (PB9217)
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Customer Q&As
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4 Customer Q&As for Anti-HOXA10 Antibody Picoband®
Question
I see that the anti-HOXA10 antibody PB9217 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus using anti-HOXA10 antibody PB9217. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-28
Question
See below the WB image, lot number and protocol we used for caudate nucleus using anti-HOXA10 antibody PB9217. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-28
Question
We are currently using anti-HOXA10 antibody PB9217 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on bovine tissues as well?
E. Zhao
Verified customer
Asked: 2014-11-21
Answer
The anti-HOXA10 antibody (PB9217) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-21