Anti-Homeodomain-only protein HOPX Antibody

Product Name

Anti-Homeodomain-only protein HOPX Antibody

View all HOP Antibodies

SKU/Catalog Number

A05019

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Homeodomain-only protein HOPX Antibody (Catalog # A05019). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

HOPX antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Homeodomain-only protein HOPX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05019)

Host

Rabbit

Contents

HOPX antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX. The immunogen is located within the first 50 amino acids of HOPX.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.

Reactive Species

A05019 is reactive to HOPX in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

8260 MW

Background of HOP

HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1). It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF)-dependent cardiac-specific gene expression and cardiac development (1). HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2,3). HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05019 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For HOPX (Source: Uniprot.org, NCBI)

Alternative Names

HOP homeobox, homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO Hopx|1110018K11Rik, 1200015P04Rik, 2300002F06Rik, AI848177, AW490897, Cam, Cameo, H, Hdop, Ho, Hod, Hop, Ob, Ob1, Obl, To, Toto|HOP homeobox|homeodomain-only protein|homeobox only domain|homeobox-only protein|mOB1|odd homeobox 1|odd homeobox protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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