PDGF-BB Platelet-Derived Growth Factor BB Mouse Recombinant Protein

Product Name

PDGF-BB Platelet-Derived Growth Factor BB Mouse Recombinant Protein

View all PDGFB recombinant proteins

SKU/Catalog Number

PROTP31240

Size

2ug, 10ug, 1mg

Description

Platelet-Derived Growth Factor BB Mouse Recombinant produced in E.coli, is a homodimeric, non-glycosylated, polypeptide chain containing 2x110 (total of 2 chains 220aa) amino acids and having a total molecular weight of 24.7 kDa. PDGF-BB is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

PDGF-BB Platelet-Derived Growth Factor BB Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP31240)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from 10mM NaCitrate pH-3.0.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Reconstitution

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml.

Reconstitution

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For PDGFB (Source: Uniprot.org, NCBI)

Alternative Names

PDGFBB; PDGF-BB PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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