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Product Info Summary
| SKU: | PROTP31240 |
|---|---|
| Size: | 2ug, 10ug, 1mg |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
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Product info
Product Name
PDGF-BB Platelet-Derived Growth Factor BB Mouse Recombinant Protein
View all PDGFB recombinant proteins
SKU/Catalog Number
PROTP31240
Size
2ug, 10ug, 1mg
Description
Platelet-Derived Growth Factor BB Mouse Recombinant produced in E.coli, is a homodimeric, non-glycosylated, polypeptide chain containing 2x110 (total of 2 chains 220aa) amino acids and having a total molecular weight of 24.7 kDa. PDGF-BB is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Cite This Product
PDGF-BB Platelet-Derived Growth Factor BB Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP31240)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from 10mM NaCitrate pH-3.0.
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Predicted MW
27.283kDa
Reconstitution
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
Biological Activity
The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For PDGFB (Source: Uniprot.org, NCBI)
Gene Name
PDGFB
Full Name
Platelet-derived growth factor subunit B
Weight
27.283kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Glioma-derived growth factor; GDGF; Osteosarcoma-derived Growth Factor; ODGF; SIS; SSV; PDGF2; c-sis; FLJ12858; PDGF-BB; PDGF B-chain; Platelet-derived growth factor beta polypeptide; Becaplermin PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PDGFB, check out the PDGFB Infographic
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In this infographic, you will see the following information for PDGFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For PDGF-BB Platelet-Derived Growth Factor BB Mouse Recombinant Protein (PROTP31240)
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