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Product Info Summary
| SKU: | A00389 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody
View all HNF-4 alpha/NR2A1 Antibodies
SKU/Catalog Number
A00389
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody catalog # A00389. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00389)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human HNF4α.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with eNOS or nNOS.
Reactive Species
A00389 is reactive to HNF4A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
52785 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00389 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of HNF4A using Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody (A00389).
Immunohistochemistry (IHC) analyzes of HNF4α/γ (K127) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 3. Immunohistochemistry validation of HNF4A using Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody (A00389).
Immunohistochemistry (IHC) analyzes of HNF4α/γ (K127) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody A00389
Western blot (WB) analysis of HNF-4α/γ (K127) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:HCT116 whole cell lysate(40ug)
Lane3:L02 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)
Lane5:AML-12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For HNF4A (Source: Uniprot.org, NCBI)
Gene Name
HNF4A
Full Name
Hepatocyte nuclear factor 4-alpha
Weight
52785 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Hepatocyte nuclear factor 4-alpha;HNF-4-alpha;Nuclear receptor subfamily 2 group A member 1;Transcription factor 14;TCF-14;Transcription factor HNF-4;HNF4A;HNF4, NR2A1, TCF14; HNF4A FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14 hepatocyte nuclear factor 4 alpha hepatocyte nuclear factor 4-alpha|HNF4alpha10/11/12|TCF-14|hepatic nuclear factor 4 alpha|nuclear receptor subfamily 2 group A member 1|transcription factor 14|transcription factor HNF-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNF4A, check out the HNF4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNF4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HNF4 Alpha / Gamma (K127) HNF4A Antibody (A00389)
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