Anti-Glucokinase/GCK Antibody Picoband®

Product Name

Anti-Glucokinase/GCK Antibody Picoband®

View all Glucokinase/GCK Antibodies

SKU/Catalog Number

A00884-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glucokinase/GCK Antibody Picoband® catalog # A00884-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glucokinase/GCK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00884-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Glucokinase recombinant protein (Position: Y234-T431).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00884-1 is reactive to GCK in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

52 kDa

Calculated molecular weight

52.191kDa

Background of Glucokinase/GCK

Glucokinase (GCK) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00884-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: rat liver tissue, mouse liver tissue

Validation Images & Assay Conditions

Gene/Protein Information For GCK (Source: Uniprot.org, NCBI)

Alternative Names

Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D; GCK GCK FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, PNDM1 glucokinase hexokinase-4|ATP:D-hexose 6-phosphotransferase|HK IV|glucokinase (hexokinase 4)|hexokinase D, pancreatic isozyme|hexokinase type IV

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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