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Product Info Summary
| SKU: | M00389 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HNF-4-alpha Rabbit Monoclonal Antibody
View all HNF-4 alpha/NR2A1 Antibodies
SKU/Catalog Number
M00389
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HNF-4-alpha Rabbit Monoclonal Antibody catalog # M00389. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF-4-alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00389)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GBA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HNF-4-alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00389 is reactive to HNF4A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
125 kDa
Calculated molecular weight
52785 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00389 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: A549 cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of HNF-4-alpha expression in A549 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using HNF-4-alpha Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HNF4A (Source: Uniprot.org, NCBI)
Gene Name
HNF4A
Full Name
Hepatocyte nuclear factor 4-alpha
Weight
52785 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Hepatocyte nuclear factor 4-alpha;HNF-4-alpha;Nuclear receptor subfamily 2 group A member 1;Transcription factor 14;TCF-14;Transcription factor HNF-4;HNF4A;HNF4, NR2A1, TCF14; HNF4A FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14 hepatocyte nuclear factor 4 alpha hepatocyte nuclear factor 4-alpha|HNF4alpha10/11/12|TCF-14|hepatic nuclear factor 4 alpha|nuclear receptor subfamily 2 group A member 1|transcription factor 14|transcription factor HNF-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNF4A, check out the HNF4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNF4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HNF-4-alpha Rabbit Monoclonal Antibody (M00389)
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No publications found for M00389
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-HNF-4-alpha Rabbit Monoclonal Antibody
Question
I have a question about product M00389, anti-HNF-4-alpha Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Krishna
Verified customer
Asked: 2020-04-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
We have been able to see staining in human liver. What should we do? Is anti-HNF-4-alpha Rabbit Monoclonal antibody supposed to stain liver positively?
J. Baker
Verified customer
Asked: 2020-02-20
Answer
From what I have seen in literature liver does express HNF4A. From what I have seen in Uniprot.org, HNF4A is expressed in liver, kidney, among other tissues. Regarding which tissues have HNF4A expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8622695
Liver, Pubmed ID: 7926813, 8964514, 24275569
Boster Scientific Support
Answered: 2020-02-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-HNF-4-alpha Rabbit Monoclonal antibody M00389. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
I was wanting to use your anti-HNF-4-alpha Rabbit Monoclonal antibody for IHC for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
You can see on the product datasheet, M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
I am interested in using your anti-HNF-4-alpha Rabbit Monoclonal antibody for sex differentiation studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thanks for your inquiry. This M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody is validated on a549 cell lysate. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The anti-HNF-4-alpha Rabbit Monoclonal antibody (M00389) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-21
Question
Our team were content with the WB result of your anti-HNF-4-alpha Rabbit Monoclonal antibody. However we have been able to see positive staining in liver nucleus. using this antibody. Is that expected? Could you tell me where is HNF4A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
From literature, liver does express HNF4A. Generally HNF4A expresses in nucleus. Regarding which tissues have HNF4A expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8622695
Liver, Pubmed ID: 7926813, 8964514, 24275569
Boster Scientific Support
Answered: 2019-07-25
Question
I see that the anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-07
Question
Is a blocking peptide available for product anti-HNF-4-alpha Rabbit Monoclonal antibody (M00389)?
M. Johnson
Verified customer
Asked: 2019-02-15
Answer
We do provide the blocking peptide for product anti-HNF-4-alpha Rabbit Monoclonal antibody (M00389). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-15
Question
Will anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 work for IHC with kidney?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
According to the expression profile of kidney, HNF4A is highly expressed in kidney. So, it is likely that anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 will work for IHC with kidney.
Boster Scientific Support
Answered: 2018-09-25
Question
Is this M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody reactive to the isotypes of HNF4A?
M. Li
Verified customer
Asked: 2016-06-16
Answer
The immunogen of M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody is A synthesized peptide derived from human HNF-4-alpha . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-06-16
Question
Would M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Jones
Verified customer
Asked: 2015-09-10
Answer
It shows on the product datasheet, M00389 anti-HNF-4-alpha Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-09-10
Question
See below the WB image, lot number and protocol we used for kidney using anti-HNF-4-alpha Rabbit Monoclonal antibody M00389. Please let me know if you require anything else.
B. Yang
Verified customer
Asked: 2015-08-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-08-06
Question
you antibody to test anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 on human kidney for research purposes, then I may be interested in using anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Anderson
Verified customer
Asked: 2015-04-07
Answer
The products we sell, including anti-HNF-4-alpha Rabbit Monoclonal antibody M00389, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-07
Question
We ordered your anti-HNF-4-alpha Rabbit Monoclonal antibody for Flow Cytometry on kidney in the past. I am using human, and We intend to use the antibody for IF next. I am interested in examining kidney as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
S. Carter
Verified customer
Asked: 2014-10-09
Answer
I took a look at the website and datasheets of our anti-HNF-4-alpha Rabbit Monoclonal antibody and it appears that M00389 has been validated on human in both Flow Cytometry and IF. Thus M00389 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-10-09
Question
Is there a BSA free version of anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 available?
G. Anderson
Verified customer
Asked: 2013-01-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HNF-4-alpha Rabbit Monoclonal antibody M00389 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-16
