Click image to see more details
Product Info Summary
| SKU: | PB9213 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Heme oxygenase 2/HMOX2 Antibody Picoband®
View all HO-2/HMOX2 Antibodies
SKU/Catalog Number
PB9213
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Heme oxygenase 2/HMOX2 Antibody Picoband® catalog # PB9213. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heme oxygenase 2/HMOX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9213)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HMOX2 recombinant protein (Position: S2-M316). Human HMOX2 shares 89% and 90% amino acid (aa) sequences identity with mouse and rat HMOX2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9213 is reactive to HMOX2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
36033 MW
Background of HO-2/HMOX2
Heme oxygenase 2 (HMOX2), also known as HO-2, is an enzyme that in humans is encoded by the HMOX2 gene. It is mapped to 16p13.3. HMOX2 belongs to the heme oxygenase family. Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9213 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human A549 whole cell, human Caco-2 whole cell, human HEK293 whole cell, human Jurkat whole cell, human HepG2 whole cell, human K562 whole cell, human Raji whole cell,
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-HMOX2 antibody, PB9213, IHC(P)
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Figure 1. Western blot analysis of HMOX2 using anti-HMOX2 antibody (PB9213).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human Raji whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX2 antigen affinity purified polyclonal antibody (Catalog # PB9213) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMOX2 at approximately 38KD. The expected band size for HMOX2 is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For HMOX2 (Source: Uniprot.org, NCBI)
Gene Name
HMOX2
Full Name
Heme oxygenase 2
Weight
36033 MW
Superfamily
heme oxygenase family
Alternative Names
Heme oxygenase 2;HO-2;1.14.99.3;HMOX2;HO2; HMOX2 HO-2 heme oxygenase 2 heme oxygenase 2|heme oxygenase (decycling) 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMOX2, check out the HMOX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMOX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heme oxygenase 2/HMOX2 Antibody Picoband® (PB9213)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Heme oxygenase 2/HMOX2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Heme oxygenase 2/HMOX2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
2 Customer Q&As for Anti-Heme oxygenase 2/HMOX2 Antibody Picoband®
Question
We are currently using anti-Heme oxygenase 2/HMOX2 antibody PB9213 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
The anti-Heme oxygenase 2/HMOX2 antibody (PB9213) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-16
Question
Has PB9213 been tested with rat samples, specifically with WB and IHC-P?
Verified customer
Asked: 2019-06-26
Answer
The Anti-Heme Oxygenase 2/HMOX2 Antibody Picoband™ (PB9213) didn't work well on rat samples.
Boster Scientific Support
Answered: 2019-07-02
