Product Info Summary
SKU: | M30947 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody
SKU/Catalog Number
M30947
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Catalog # M30947). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30947)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM289
Isotype
IgG2b, kappa
Immunogen
Activated human peripheral blood mononuclear cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30947 is reactive to HLA-DR in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
29966 MW
Background of HLA-DRA
This monoclonal antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30947 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-HLA-DR Monoclonal Antibody (SPM289).
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Western Blot analysis of Ramos cells and human spleen tissue lysate using Anti-HLA-DR Monoclonal Antibody (SPM289).
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Immunofluorescence analysis of Raji cells labeling HLA-DR with Anti-HLA-DR Monoclonal Antibody (SPM289) conjugated with APC (Green). The nuclear counterstain is Reddot (Red).
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Flow Cytometric analysis of human Raji cells using Anti-HLA-DR Monoclonal Antibody (SPM289) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Target Info & Infographic
Gene/Protein Information For HLA-DR (Source: Uniprot.org, NCBI)
Gene Name
HLA-DR
Full Name
HLA class II histocompatibility antigen, DR alpha chain
Weight
29966 MW
Superfamily
MHC class II family
Alternative Names
HLA class II histocompatibility antigen, DRB1-15 beta chain;DW2.2/DR2.2;MHC class II antigen DRB1*15;HLA-DRB1;HLA-DRB2; HLA-DRA HLA-DRA1 major histocompatibility complex, class II, DR alpha HLA class II histocompatibility , DR alpha chain|MHC class II DRA|histocompatibility HLA-DR alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-DR, check out the HLA-DR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-DR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (M30947)
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No publications found for M30947
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Customer Q&As
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5 Customer Q&As for Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-HLA-DRB (MHC II) Monoclonal antibody M30947. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-22
Question
Can you help my question with product M30947, anti-HLA-DRB (MHC II) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30947 anti-HLA-DRB (MHC II) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-21
Question
Is a blocking peptide available for product anti-HLA-DRB (MHC II) Monoclonal antibody (M30947)?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
We do provide the blocking peptide for product anti-HLA-DRB (MHC II) Monoclonal antibody (M30947). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-08
Question
We need to test anti-HLA-DRB (MHC II) Monoclonal antibody M30947 on human peripheral blood for research purposes, then I may be interested in using anti-HLA-DRB (MHC II) Monoclonal antibody M30947 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Zhang
Verified customer
Asked: 2017-09-25
Answer
The products we sell, including anti-HLA-DRB (MHC II) Monoclonal antibody M30947, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-25
Question
Will anti-HLA-DRB (MHC II) Monoclonal antibody M30947 work on dog IHC with small intestine?
T. Bhatt
Verified customer
Asked: 2016-01-22
Answer
Our lab technicians have not validated anti-HLA-DRB (MHC II) Monoclonal antibody M30947 on dog. You can run a BLAST between dog and the immunogen sequence of anti-HLA-DRB (MHC II) Monoclonal antibody M30947 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog small intestine in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-22