Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody

Product Name

Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody

View all HLA-DRA Antibodies

SKU/Catalog Number

M30947

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Catalog # M30947). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30947)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM289

Isotype

IgG2b, kappa

Immunogen

Activated human peripheral blood mononuclear cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M30947 is reactive to HLA-DRA in Human

Applications

M30947 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29966 MW

Background of HLA-DRA

This monoclonal antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-HLA-DR Monoclonal Antibody (SPM289).

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Western Blot analysis of Ramos cells and human spleen tissue lysate using Anti-HLA-DR Monoclonal Antibody (SPM289).

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Immunofluorescence analysis of Raji cells labeling HLA-DR with Anti-HLA-DR Monoclonal Antibody (SPM289) conjugated with APC (Green). The nuclear counterstain is Reddot (Red).

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Flow Cytometric analysis of human Raji cells using Anti-HLA-DR Monoclonal Antibody (SPM289) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For HLA-DRA (Source: Uniprot.org, NCBI)

Alternative Names

FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW HLA-DRA HLA-DRA1 major histocompatibility complex, class II, DR alpha HLA class II histocompatibility antigen, DR alpha chain|MHC class II antigen DRA|histocompatibility antigen HLA-DR alpha

For more info on HLA-DRA, check out the HLA-DRA Infographic

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In this infographic, you will see the following information for HLA-DRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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