Anti-HLA-DR Antibody

Product Name

Anti-HLA-DR Antibody

View all HLA-DRA Antibodies

SKU/Catalog Number

A01195-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-HLA-DR Antibody catalog # A01195-2. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA-DR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01195-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Op18 around the phosphorylation site of S62.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01195-2 is reactive to HLA-DRA in Human, Mouse, Rat

Applications

A01195-2 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

28607 MW

Background of HLA-DRA

Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.

Sato N., EMBO J. 16:4340-4351(1997).
Hess G.F., Gene 211:133-140(1998).
Jiang W., Proc. Natl. Acad. Sci. U.S.A. 94:14320-14325(1997).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of HLA-DRA using Anti-HLA-DR Antibody (A01195-2).

ICC staining HLA-DR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-HLA-DR Antibody A01195-2

Western blot analysis of HLA-DR on Daudi cells lysates using anti-HLA-DR antibody at 1/1

Gene/Protein Information For HLA-DRA (Source: Uniprot.org, NCBI)

Alternative Names

FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW HLA-DRA HLA-DRA1 major histocompatibility complex, class II, DR alpha HLA class II histocompatibility antigen, DR alpha chain|MHC class II antigen DRA|histocompatibility antigen HLA-DR alpha

For more info on HLA-DRA, check out the HLA-DRA Infographic

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In this infographic, you will see the following information for HLA-DRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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