Anti-HLA-DR/HLA-DRA Antibody Picoband®

HLA-DRA antibody

Boster Bio Anti-HLA-DR/HLA-DRA Antibody Picoband® catalog # A01195. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A01195
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, WB

Product Name

Anti-HLA-DR/HLA-DRA Antibody Picoband®

View all HLA-DRA Antibodies

SKU/Catalog Number

A01195

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA-DR/HLA-DRA Antibody Picoband® catalog # A01195. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA-DR/HLA-DRA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01195)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HLA-DR/HLA-DRA recombinant protein (Position: I26-L254).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01195 is reactive to HLA-DRA in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

82536 MW

Background of HLA-DRA

HLA class II histocompatibility antigen, DR alpha chain is a protein that in humans is encoded by the HLA-DRA gene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01195 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human Raji whole cell, human Daudi whole cell, human Ramos whole cell, human HMy2CIR whole cell
IHC: human mammary cancer tissue, human mammary cancer tissue, human duodenal adenocarcinoma tissue, human laryngeal squamous cell carcinoma tissue, human seminoma testis tissue
FCM: human PBMC cell, SW620 cell

Validation Images & Assay Conditions

Gene/Protein Information For HLA-DRA (Source: Uniprot.org, NCBI)

Gene Name

HLA-DRA

Full Name

HLA class II histocompatibility antigen, DR alpha chain

Weight

82536 MW

Superfamily

MHC class II family

Alternative Names

HLA class II histocompatibility antigen, DR alpha chain; MHC class II antigen DRA; HLA-DRA; HLA-DRA1 HLA-DRA HLA-DRA1 major histocompatibility complex, class II, DR alpha HLA class II histocompatibility , DR alpha chain|MHC class II DRA|histocompatibility HLA-DR alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HLA-DRA, check out the HLA-DRA Infographic

HLA-DRA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HLA-DRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01195 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shuyuan Guo,Yusen Zhang,Yanmin Zhang,Fanhua Meng,Minghua Li,Zhendong Yu,Yun Chen,Guanghui Cui, "Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute
Species: Human,Rat
A01195 usage in article: APP:FLOW CYTOMETRY, SAMPLE:PBMC-5 CELL, DILUTION:NA

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1 Customer Q&As for Anti-HLA-DR/HLA-DRA Antibody Picoband®

Question

We are currently using anti-HLA-DR/HLA-DRA antibody A01195 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

K. Walker

Verified customer

Asked: 2019-06-26

Answer

The anti-HLA-DR/HLA-DRA antibody (A01195) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-26

Order DetailsPrice
A01195

100μg

$370
A01195-10ug

10μg sample (liquid)

$99
A01195-Biotin

100 μg Biotin conjugated

$570
A01195-Cy3

100 μg Cy3 conjugated

$570
A01195-Dylight488

100 μg Dylight488 conjugated

$570
A01195-Dylight550

100 μg Dylight550 conjugated

$570
A01195-Dylight594

100 μg Dylight594 conjugated

$570
A01195-FITC

100 μg FITC conjugated

$570
A01195-HRP

100 μg HRP conjugated

$570
A01195-APC

100 μg APC conjugated

$670
A01195-PE

100 μg PE conjugated

$670
A01195-iFluor647

100 μg iFluor647 conjugated

$670
A01195-carrier-free

Carrier Free

$370

More conjugates/formats

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A01195
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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