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Product Info Summary
| SKU: | A07815 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HLA-DOB/Hla Dob Antibody
SKU/Catalog Number
A07815
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HLA-DOB/Hla Dob Antibody (Catalog # A07815). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-DOB/Hla Dob Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07815)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human HLA-DOB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07815 is reactive to HLA-DOB in Human
Reconstitution
Calculated molecular weight
30822 MW
Background of HLA DOB
Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.
Tonnelle C., EMBO J. 4:2839-2847(1985).
Jonsson A.-K., Immunogenetics 29:411-413(1989).
Beck S., J. Mol. Biol. 228:433-441(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07815 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cell, using HLA-DOB antibody A07815.
Protein Target Info & Infographic
Gene/Protein Information For HLA-DOB (Source: Uniprot.org, NCBI)
Gene Name
HLA-DOB
Full Name
HLA class II histocompatibility antigen, DO beta chain
Weight
30822 MW
Superfamily
MHC class II family
Alternative Names
HLA class II histocompatibility antigen, DO beta chain;MHC class II antigen DOB;HLA-DOB; HLA-DOB DOB, HLA_DOB major histocompatibility complex, class II, DO beta HLA class II histocompatibility , DO beta chain|MHC class II DOB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-DOB, check out the HLA-DOB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-DOB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-DOB/Hla Dob Antibody (A07815)
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No publications found for A07815
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-HLA-DOB/Hla Dob Antibody
Question
Do you have a BSA free version of anti-HLA-DOB/Hla Dob antibody A07815 available?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLA-DOB/Hla Dob antibody A07815 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-HLA-DOB/Hla Dob antibody (A07815)?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
We do provide the blocking peptide for product anti-HLA-DOB/Hla Dob antibody (A07815). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-25
Question
We are currently using anti-HLA-DOB/Hla Dob antibody A07815 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
The anti-HLA-DOB/Hla Dob antibody (A07815) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-13
Question
I see that the anti-HLA-DOB/Hla Dob antibody A07815 works with WB, what is the protocol used to produce the result images on the product page?
M. Zhang
Verified customer
Asked: 2018-05-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-11
Question
I was wanting to use your anti-HLA-DOB/Hla Dob antibody for WB for human small intestine peyer's patch on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine peyer's patch identification?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
You can see on the product datasheet, A07815 anti-HLA-DOB/Hla Dob antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine peyer's patch in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-20
Question
Does anti-HLA-DOB/Hla Dob antibody A07815 work for WB with small intestine peyer's patch?
K. Johnson
Verified customer
Asked: 2017-12-15
Answer
According to the expression profile of small intestine peyer's patch, HLA-DOB is highly expressed in small intestine peyer's patch. So, it is likely that anti-HLA-DOB/Hla Dob antibody A07815 will work for WB with small intestine peyer's patch.
Boster Scientific Support
Answered: 2017-12-15
