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Product Info Summary
| SKU: | A04817 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-HAIR HR Antibody
SKU/Catalog Number
A04817
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HAIR HR Antibody (Catalog # A04817). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HAIR HR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04817)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human HAIR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04817 is reactive to HR in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
127495 MW
Background of Hairless
Histone demethylase that specifically demethylates both mono- and dimethylated 'Lys-9' of histone H3. May act as a transcription regulator controlling hair biology (via targeting of collagens), neural activity, and cell cycle.
Ahmad W., Science 279:720-724(1998).
Cichon S., Hum. Mol. Genet. 7:1671-1679(1998).
Ahmad W., Am. J. Hum. Genet. 63:984-991(1998).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04817 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HAIR antibody A04817.
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Western blot analysis of extracts from 293 cells, using HAIR antibody A04817.
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Immunofluorescence analysis of A549 cells, using HAIR antibody A04817.
Protein Target Info & Infographic
Gene/Protein Information For HR (Source: Uniprot.org, NCBI)
Gene Name
HR
Full Name
Lysine-specific demethylase hairless
Weight
127495 MW
Alternative Names
Lysine-specific demethylase hairless;1.14.11.-;HR; Hr|ALUNC, b, bld, bldy, hr, r, rh-bm, rh-bmh|lysine demethylase and nuclear receptor corepressor|lysine-specific demethylase hairless|[histone H3]-dimethyl-L-lysine(9) demethylase hairless|hairless|protein hairless
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HR, check out the HR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HAIR HR Antibody (A04817)
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No publications found for A04817
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-HAIR HR Antibody
Question
We are currently using anti-HAIR antibody A04817 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The anti-HAIR antibody (A04817) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
I was wanting to use to test anti-HAIR antibody A04817 on human eye for research purposes, then I may be interested in using anti-HAIR antibody A04817 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Krishna
Verified customer
Asked: 2020-04-01
Answer
The products we sell, including anti-HAIR antibody A04817, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-01
Question
Will A04817 anti-HAIR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
It shows on the product datasheet, A04817 anti-HAIR antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-04
Question
I was wanting to use your anti-HAIR antibody for IHC for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can see on the product datasheet, A04817 anti-HAIR antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-19
Question
Can you help my question with product A04817, anti-HAIR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04817 anti-HAIR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-05
Question
Is this A04817 anti-HAIR antibody reactive to the isotypes of HR?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The immunogen of A04817 anti-HAIR antibody is Synthesized peptide derived from internal of human HAIR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-HAIR antibody A04817. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
Is a blocking peptide available for product anti-HAIR antibody (A04817)?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We do provide the blocking peptide for product anti-HAIR antibody (A04817). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-29
Question
I see that the anti-HAIR antibody A04817 works with IHC, what is the protocol used to produce the result images on the product page?
P. Lewis
Verified customer
Asked: 2019-06-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-21
Question
Does anti-HAIR antibody A04817 work on goat IF with fetal brain peripheral blood leukocyte?
G. Carter
Verified customer
Asked: 2018-10-18
Answer
Our lab technicians have not validated anti-HAIR antibody A04817 on goat. You can run a BLAST between goat and the immunogen sequence of anti-HAIR antibody A04817 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat fetal brain peripheral blood leukocyte in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-18
Question
Would anti-HAIR antibody A04817 work for IHC with eye?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
According to the expression profile of eye, HR is highly expressed in eye. So, it is likely that anti-HAIR antibody A04817 will work for IHC with eye.
Boster Scientific Support
Answered: 2018-07-06
Question
Is there a BSA free version of anti-HAIR antibody A04817 available?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HAIR antibody A04817 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-26
Question
Here is the WB image, lot number and protocol we used for eye using anti-HAIR antibody A04817. Please let me know if you require anything else.
Z. Yang
Verified customer
Asked: 2015-07-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-28
