Click image to see more details
Product Info Summary
| SKU: | EK1935 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FCRL5 ELISA Kit PicoKine®
View all FCRL5/FcRH5/IRTA2 ELISA kits
SKU/Catalog Number
EK1935
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FCRL5 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCRL5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FCRL5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1935)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q16-R844
Sensitivity
<0.1 ng/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1935 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1935 is reactive to FCRL5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1935 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRL5/FcRH5/IRTA2
Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1935-CAP | Anti-Human FCRL5 Pre-coated 96-well strip microplate | 1 |
| EK1935-ST | Human FCRL5 Standard | 2 vials, 50 ng/tube |
| EK1935-DA | Human FCRL5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.012 | 0.109 | 0.141 | 0.258 | 0.593 | 1.007 | 1.144 | 1.880 |
Data Example Images
Click image to see more details
Human FCRL5 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCRL5/FcRH5/IRTA2, Dilution ratio of 1:5, concentration in serum and plasma is around 50 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FCRL5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1090 | 6877 | 21168 | 1262 | 6894 | 22254 |
| Standard deviation | 56.68 | 433.25 | 1016.06 | 61.84 | 510.16 | 1179.46 |
| CV (%) | 5.2% | 6.3% | 4.8% | 4.9% | 7.4% | 5.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCRL5/FcRH5/IRTA2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1090 | 1154 | 1262 | 1129 | 1158 | 63.82 | 5.5% |
| Sample 2 | 6877 | 7371 | 6834 | 7977 | 7264 | 462.19 | 6.3% |
| Sample 3 | 21168 | 22254 | 20007 | 18723 | 20538 | 1315.08 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For FCRL5 (Source: Uniprot.Org, NCBI)
Gene Name
FCRL5
Full Name
Fc receptor-like protein 5
Weight
106.437kDa
Alternative Names
FcR-like protein 5; FcRL5; BXMAS1; Fc receptor homolog 5; FcRH5; Immune receptor translocation-associated protein 2; CD307e; FCRH5; IRTA2; UNQ503/PRO820 FCRL5 BXMAS1, CD307, CD307e, FCRH5, IRTA2, PRO820 Fc receptor like 5 Fc receptor-like protein 5|fc receptor homolog 5|fcR-like protein 5|immune receptor translocation-associated protein 2|immunoglobulin superfamily receptor translocation associated 2 (IRTA2)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCRL5, check out the FCRL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCRL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FCRL5 ELISA Kit PicoKine® (EK1935)
Hello CJ!
No publications found for EK1935
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human FCRL5 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human FCRL5 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Human FCRL5 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-12-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-12-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-05-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-05-24
Question
Q: how to analyze ELISA data? I have performed FCRL5 level in plasma.
Verified Customer
Verified customer
Asked: 2019-11-02
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-02
Question
Q: Can FCRL5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
A. Wu
Verified customer
Asked: 2019-10-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-10-14
Question
Q: What is the optimal O.D. value for FCRL5 ELISA kit? I used your FCRL5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain FCRL5 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FCRL5 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of FCRL5 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2019-04-17
Question
Q: how to thaw whole blood sample for FCRL5 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FCRL5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-02-18
Question
Q: if the enzyme conjugated FCRL5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FCRL5 antigen?
D. Miller
Verified customer
Asked: 2019-02-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-05
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis FCRL5 using the EK1935 Human FCRL5 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-05-21
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
T. Chen
Verified customer
Asked: 2018-03-26
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-26
