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Product Info Summary
| SKU: | A30721 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-HLA-DOA/Hla Doa Antibody
SKU/Catalog Number
A30721
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HLA-DOA/Hla Doa Antibody (Catalog# A30721). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-DOA/Hla Doa Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30721)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HLA-DOA. Immunogen sequence location: 71-120
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30721 is reactive to HLA-DOA in Human
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
27599 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30721 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from COLO cells, using HLA-DOA Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For HLA-DOA (Source: Uniprot.org, NCBI)
Gene Name
HLA-DOA
Full Name
HLA class II histocompatibility antigen, DO alpha chain
Weight
27599 MW
Superfamily
MHC class II family
Alternative Names
HLA-DOA;HLA-DNA;HLA-DZA;HLA class II histocompatibility antigen; DO alpha chain;MHC DN-alpha;MHC DZ alpha;MHC class II antigen DOA HLA-DOA HLA-DNA, HLA-DZA, HLADZ major histocompatibility complex, class II, DO alpha HLA class II histocompatibility , DO alpha chain|HLA-D0-alpha|MHC DN-alpha|MHC DZ alpha|MHC class II DOA|lymphocyte |major histocompatibility complex, class II, DN alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-DOA, check out the HLA-DOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-DOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA-DOA/Hla Doa Antibody (A30721)
Hello CJ!
No publications found for A30721
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-HLA-DOA/Hla Doa Antibody
Question
Can you help my question with product A30721, anti-HLA-DOA/Hla Doa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Jones
Verified customer
Asked: 2020-03-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30721 anti-HLA-DOA/Hla Doa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-11
Question
Does anti-HLA-DOA/Hla Doa antibody A30721 work for WB with amniotic fluid?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
According to the expression profile of amniotic fluid, HLA-DOA is highly expressed in amniotic fluid. So, it is likely that anti-HLA-DOA/Hla Doa antibody A30721 will work for WB with amniotic fluid.
Boster Scientific Support
Answered: 2020-03-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-HLA-DOA/Hla Doa antibody A30721. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-11
Question
My lab would like to test anti-HLA-DOA/Hla Doa antibody A30721 on human amniotic fluid for research purposes, then I may be interested in using anti-HLA-DOA/Hla Doa antibody A30721 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Jha
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-HLA-DOA/Hla Doa antibody A30721, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
We are currently using anti-HLA-DOA/Hla Doa antibody A30721 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
The anti-HLA-DOA/Hla Doa antibody (A30721) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-04
Question
Is a blocking peptide available for product anti-HLA-DOA/Hla Doa antibody (A30721)?
B. Kulkarni
Verified customer
Asked: 2018-08-06
Answer
We do provide the blocking peptide for product anti-HLA-DOA/Hla Doa antibody (A30721). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-06
