Anti-HLA A/HLA-A Antibody Picoband®

Product Name

Anti-HLA A/HLA-A Antibody Picoband®

View all HLA-A Antibodies

SKU/Catalog Number

PB9376

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA A/HLA-A Antibody Picoband® catalog # PB9376. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA A/HLA-A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9376)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HLA A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9376 is reactive to HLA-A in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

41 kDa

Calculated molecular weight

40922 MW

Background of HLA-A

HLA-A, namely HLA A, is a group of human leukocyte antigens (HLA) that are coded for by the HLA-A locus, which is located at human chromosome 6p21.3. HLA is simply the major histocompatibility complex (MHC) specific to humans. HLA-A is one of three major types of human MHC class I cell surface receptors. The others are HLA-B and HLA-C. And it is critical to the cytotoxic t-cell controlled immune response to viruses and other intracellular pathogens. Because each HLA-A gene has a high affinity for slightly different peptides, certain HLA-As are associated with increased risk, more rapid progression, and/or increased severity of many diseases. For similar reasons, HLA-A matching is essential to successful tissue transplants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9376 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Positive Control

WB: human U87 whole cell, human HL-60 whole cell, human A431 whole cell, human HEL whole cell
IHC: Human Intestinal Cancer tissue
ICC/IF: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For HLA-A (Source: Uniprot.org, NCBI)

Alternative Names

HLA class I histocompatibility antigen, A-2 alpha chain;MHC class I antigen A*2;HLA-A;HLAA; HLA-A HLAA major histocompatibility complex, class I, A HLA class I histocompatibility , A alpha chain|HLA class I histocompatibility , A-1 alpha chain|MHC class I HLA-A heavy chain|leukocyte class I-A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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