Product Info Summary
SKU: | M12477 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody
SKU/Catalog Number
M12477
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody catalog # M12477. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IHA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12477 is reactive to HIST1H3A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
15404 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12477 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:50
Positive Control
WB: HeLa cell, NIH/3T3 cell, Rat brain
IHC: Human squamous cell carcinoma tissue, Rat cerebral cortex tissue, Mouse hippocampus tissue, Rat pancreas tissue, Human cervical cancer tissue, human colon tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate.
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Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse hippocampus , using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon, using Histone H3 Antibody.
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Immunofluorescent analysis of HeLa cells, using Histone H3 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HIST1H3A (Source: Uniprot.org, NCBI)
Gene Name
HIST1H3A
Full Name
Histone H3.1
Weight
15404 MW
Superfamily
histone H3 family
Alternative Names
Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ; H3C1 H3/A0, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3C1 H3 clustered histone 1 histone H3.1|H3 histone family, member A|histone 1, H3a|histone H3/a|histone cluster 1 H3 family member a|histone cluster 1, H3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H3A, check out the HIST1H3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody (M12477)
Hello CJ!
M12477 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zileuton ameliorates depressive-like behaviors, hippocampal neuroinflammation, apoptosis and synapse dysfunction in mice exposed to chronic mild stress
Antiproliferative and proapoptotic effects of somatostatin on activated hepatic stellate cells
Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose
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