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Product Info Summary
| SKU: | A33947S10 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Histone H3 (S10) Antibody
SKU/Catalog Number
A33947S10
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H3 (S10) Antibody catalog # A33947S10. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H3 (S10) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33947S10)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A33947S10 is reactive to H3C1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
25096 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33947S10 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1, Histone H3 (Phospho- Ser10) polyclonal antibody (red) was diluted at 1:200 (4° overnight). β-Tubulin Monoclonal antibody (5G3) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Western Blot analysis of various cells using Phospho-Histone H3 (S10) polyclonal antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western Blot analysis of KB cells using Phospho-Histone H3 (S10) polyclonal antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Histone H3.1 (Phospho-Ser10) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells and HepG2 cells, using Histone H3.1 (Phospho-Ser10) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For H3C1 (Source: Uniprot.org, NCBI)
Gene Name
H3C1
Full Name
Histone H3.1
Weight
25096 MW
Superfamily
histone H3 family
Alternative Names
ATP synthase subunit a;F-ATPase protein 6;Mtatp6;Atp6, Atpase6, mt-Atp6; H3C1 H3/A0, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3C1 H3 clustered histone 1 histone H3.1|H3 histone family, member A|histone 1, H3a|histone H3/a|histone cluster 1 H3 family member a|histone cluster 1, H3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H3C1, check out the H3C1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H3C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Histone H3 (S10) Antibody (A33947S10)
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