Product Info Summary
SKU: | M02263 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody
View all ARNT/HIF-1 beta Antibodies
SKU/Catalog Number
M02263
BM4377 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody catalog # M02263. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02263)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EAD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HIF-1 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02263 is reactive to ARNT in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
16 kDa
Calculated molecular weight
86636 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02263 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:500-1:2000
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using HIF-1 beta Antibody .
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of HIF-1 beta expression in Human fetal kidney lysate.
Protein Target Info & Infographic
Gene/Protein Information For ARNT (Source: Uniprot.org, NCBI)
Gene Name
ARNT
Full Name
Aryl hydrocarbon receptor nuclear translocator
Weight
86636 MW
Alternative Names
Aryl hydrocarbon receptor nuclear translocator;ARNT protein;Class E basic helix-loop-helix protein 2;bHLHe2;Dioxin receptor, nuclear translocator;Hypoxia-inducible factor 1-beta;HIF-1-beta;HIF1-beta;ARNT;BHLHE2; ARNT HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2 aryl hydrocarbon receptor nuclear translocator aryl hydrocarbon receptor nuclear translocator|class E basic helix-loop-helix protein 2|dioxin receptor, nuclear translocator|hypoxia-inducible factor 1, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARNT, check out the ARNT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARNT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody (M02263)
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No publications found for M02263
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Customer Q&As
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6 Customer Q&As for Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody
Question
Does anti-HIF-1 beta Rabbit Monoclonal antibody M02263 work for WB with thalamus uterus?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
According to the expression profile of thalamus uterus, ARNT is highly expressed in thalamus uterus. So, it is likely that anti-HIF-1 beta Rabbit Monoclonal antibody M02263 will work for WB with thalamus uterus.
Boster Scientific Support
Answered: 2020-04-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus uterus using anti-HIF-1 beta Rabbit Monoclonal antibody M02263. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
I see that the anti-HIF-1 beta Rabbit Monoclonal antibody M02263 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-08
Question
Will anti-HIF-1 beta Rabbit Monoclonal antibody M02263 work on canine IHC with brain?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
Our lab technicians have not tested anti-HIF-1 beta Rabbit Monoclonal antibody M02263 on canine. You can run a BLAST between canine and the immunogen sequence of anti-HIF-1 beta Rabbit Monoclonal antibody M02263 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-11
Question
Is there a BSA free version of anti-HIF-1 beta Rabbit Monoclonal antibody M02263 available?
F. Jha
Verified customer
Asked: 2016-05-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HIF-1 beta Rabbit Monoclonal antibody M02263 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-24
Question
Is this M02263 anti-HIF-1 beta Rabbit Monoclonal antibody reactive to the isotypes of ARNT?
V. Carter
Verified customer
Asked: 2013-05-17
Answer
The immunogen of M02263 anti-HIF-1 beta Rabbit Monoclonal antibody is A synthesized peptide derived from human HIF-1 beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-05-17