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Product Info Summary
| SKU: | A04030 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-USF2 Antibody
SKU/Catalog Number
A04030
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-USF2 Antibody (Catalog # A04030). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04030)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human USF-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04030 is reactive to USF2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
36955 MW
Background of USF2
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Nobuto Shibata, J. Gerontol. A Biol. Sci. Med. Sci., Jul 2006; 61: 660 - 662.
J L Meier, Mol. Cell. Biol., Oct 1994; 14: 6896 - 6906.
Hassan Sellak, J. Biol. Chem., May 2005; 280: 18425 - 18433.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04030 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells and HepG2 cells using USF2 antibody A04030. The lane on the left is treated with synthesized peptide.
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Western blot analysis of extracts from HeLa cells, using USF2 antibody A04030.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using USF2 antibody A04030.
Protein Target Info & Infographic
Gene/Protein Information For USF2 (Source: Uniprot.org, NCBI)
Gene Name
USF2
Full Name
Upstream stimulatory factor 2
Weight
36955 MW
Alternative Names
Upstream stimulatory factor 2;Class B basic helix-loop-helix protein 12;bHLHb12;FOS-interacting protein;FIP;Major late transcription factor 2;Upstream transcription factor 2;USF2;BHLHB12; USF2 FIP, bHLHb12 upstream transcription factor 2, c-fos interacting upstream stimulatory factor 2|c-fos interacting protein|class B basic helix-loop-helix protein 12|major late transcription factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USF2, check out the USF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USF2 Antibody (A04030)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-USF2 Antibody
Question
We are currently using anti-USF2 antibody A04030 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-USF2 antibody (A04030) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07
Question
Will anti-USF2 antibody A04030 work on primate WB with b-cell?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Our lab technicians have not validated anti-USF2 antibody A04030 on primate. You can run a BLAST between primate and the immunogen sequence of anti-USF2 antibody A04030 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
Is this A04030 anti-USF2 antibody reactive to the isotypes of USF2?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The immunogen of A04030 anti-USF2 antibody is Synthesized peptide derived from Internal of human USF-2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-04
Question
Do you have a BSA free version of anti-USF2 antibody A04030 available?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-USF2 antibody A04030 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-21
Question
Is a blocking peptide available for product anti-USF2 antibody (A04030)?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
We do provide the blocking peptide for product anti-USF2 antibody (A04030). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-29
Question
Will A04030 anti-USF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
It shows on the product datasheet, A04030 anti-USF2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-04
Question
See below the WB image, lot number and protocol we used for b-cell using anti-USF2 antibody A04030. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-18
Question
Would anti-USF2 antibody A04030 work for WB with b-cell?
R. Parker
Verified customer
Asked: 2018-02-15
Answer
According to the expression profile of b-cell, USF2 is highly expressed in b-cell. So, it is likely that anti-USF2 antibody A04030 will work for WB with b-cell.
Boster Scientific Support
Answered: 2018-02-15
Question
We need to test anti-USF2 antibody A04030 on mouse b-cell for research purposes, then I may be interested in using anti-USF2 antibody A04030 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
The products we sell, including anti-USF2 antibody A04030, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-24
Question
I was wanting to use your anti-USF2 antibody for WB for mouse b-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse b-cell identification?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
As indicated on the product datasheet, A04030 anti-USF2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse b-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-USF2 antibody A04030. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-21
Question
Can you help my question with product A04030, anti-USF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Lewis
Verified customer
Asked: 2016-08-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04030 anti-USF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-17
Question
I see that the anti-USF2 antibody A04030 works with WB, what is the protocol used to produce the result images on the product page?
W. Dhar
Verified customer
Asked: 2014-09-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-03
