Click image to see more details
Product Info Summary
| SKU: | PB9211 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HEXB Antibody Picoband®
SKU/Catalog Number
PB9211
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HEXB Antibody Picoband® catalog # PB9211. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEXB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9211)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9211 is reactive to HEXB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
63 kDa
Calculated molecular weight
63111 MW
Background of HEXB
Beta-hexosaminidase subunit beta (HEXB) is an enzyme that in humans is encoded by the HEXB gene. It is mapped to 5q13.3. HEXB is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). It has been found that HEXB is a peptidoglycan hydrolase and it is involved in restricting mycobacteria growth even before the onset of adaptive immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9211 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U87 whole cell
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HEXB using anti-HEXB antibody (PB9211).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXB antigen affinity purified polyclonal antibody (Catalog # PB9211) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXB at approximately 63 kDa. The expected band size for HEXB is at 63 kDa.
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Anti-HEXB antibody, PB9211,IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For HEXB (Source: Uniprot.org, NCBI)
Gene Name
HEXB
Full Name
Beta-hexosaminidase subunit beta
Weight
63111 MW
Superfamily
glycosyl hydrolase 20 family
Alternative Names
Beta-hexosaminidase subunit beta;3.2.1.52;Beta-N-acetylhexosaminidase subunit beta;Hexosaminidase subunit B;Cervical cancer proto-oncogene 7 protein;HCC-7;N-acetyl-beta-glucosaminidase subunit beta;Beta-hexosaminidase subunit beta chain B;Beta-hexosaminidase subunit beta chain A;HEXB;HCC7; HEXB ENC-1AS, HEL-248, HEL-S-111 hexosaminidase subunit beta beta-hexosaminidase subunit beta|HCC-7|N-acetyl-beta-glucosaminidase subunit beta|beta-N-acetylhexosaminidase subunit beta|beta-hexosaminidase beta-subunit|cervical cancer proto-oncogene 7 protein|epididymis luminal protein 248|epididymis secretory protein Li 111|epididymis secretory sperm binding protein|hexosaminidase B (beta polypeptide)|hexosaminidase subunit B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HEXB, check out the HEXB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HEXB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HEXB Antibody Picoband® (PB9211)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-HEXB Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for liver using anti-HEXB antibody PB9211. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
Will anti-HEXB antibody PB9211 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
According to the expression profile of liver, HEXB is highly expressed in liver. So, it is likely that anti-HEXB antibody PB9211 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-12-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-HEXB antibody PB9211. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-25
Question
I would like to test anti-HEXB antibody PB9211 on human liver for research purposes, then I may be interested in using anti-HEXB antibody PB9211 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The products we sell, including anti-HEXB antibody PB9211, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-27
Question
I see that the anti-HEXB antibody PB9211 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-05
Question
Do you have a BSA free version of anti-HEXB antibody PB9211 available?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HEXB antibody PB9211 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-07
Question
We are currently using anti-HEXB antibody PB9211 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
J. Collins
Verified customer
Asked: 2014-12-08
Answer
The anti-HEXB antibody (PB9211) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-08
