HEXB Colorimetric Cell-Based ELISA

HEXB ELISA kit, cell-based

The HEXB Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HEXB protein expression profile in cells. The kit can be used for measuring the relative amounts of HEXB in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HEXB.

Product Info Summary

SKU: EKC1671
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human
Application: ELISA

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Product Name

HEXB Colorimetric Cell-Based ELISA

See all HEXB products

SKU/Catalog Number

EKC1671

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The HEXB Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HEXB protein expression profile in cells. The kit can be used for measuring the relative amounts of HEXB in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HEXB.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

HEXB Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1671)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1671 is reactive to HEXB in Human samples

Application Guarantee

EKC1671 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-HEXB Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For HEXB (Source: Uniprot.Org, NCBI)

Gene Name

HEXB

Full Name

Beta-hexosaminidase subunit beta

Weight

63111 MW

Superfamily

glycosyl hydrolase 20 family

Alternative Names

Beta-hexosaminidase subunit beta;3.2.1.52;Beta-N-acetylhexosaminidase subunit beta;Hexosaminidase subunit B;Cervical cancer proto-oncogene 7 protein;HCC-7;N-acetyl-beta-glucosaminidase subunit beta;Beta-hexosaminidase subunit beta chain B;Beta-hexosaminidase subunit beta chain A;HEXB;HCC7; HEXB ENC-1AS, HEL-248, HEL-S-111 hexosaminidase subunit beta beta-hexosaminidase subunit beta|HCC-7|N-acetyl-beta-glucosaminidase subunit beta|beta-N-acetylhexosaminidase subunit beta|beta-hexosaminidase beta-subunit|cervical cancer proto-oncogene 7 protein|epididymis luminal protein 248|epididymis secretory protein Li 111|epididymis secretory sperm binding protein|hexosaminidase B (beta polypeptide)|hexosaminidase subunit B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HEXB, check out the HEXB Infographic

HEXB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HEXB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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