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Product Info Summary
| SKU: | M05384 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-HES5 Monoclonal Antibody
SKU/Catalog Number
M05384
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HES5 Monoclonal Antibody catalog # M05384. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HES5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05384)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEGI-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HES5 Transcriptional repressor of genes that require a bHLH protein for their transcription.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05384 is reactive to HES5 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
26 kDa
Calculated molecular weight
95926 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05384 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of HES5 expression in (1) 293 cell lysate; (2) Mouse spleen lysate; (3) C6 cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For HES5 (Source: Uniprot.org, NCBI)
Gene Name
HES5
Full Name
Transcription factor HES-5
Weight
95926 MW
Alternative Names
ATP-binding cassette sub-family F member 1;ATP-binding cassette 50;TNF-alpha-stimulated ABC protein;ABCF1;ABC50; Hes5|bHLHb3, bHLHb38|hes family bHLH transcription factor 5|transcription factor HES-5|hairy and enhancer of split 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HES5, check out the HES5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HES5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HES5 Monoclonal Antibody (M05384)
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No publications found for M05384
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Customer Q&As
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5 Customer Q&As for Anti-HES5 Monoclonal Antibody
Question
Does anti-HES5 Monoclonal antibody M05384 work for WB with dorsolateral prefrontal cortex?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
According to the expression profile of dorsolateral prefrontal cortex, HES5 is highly expressed in dorsolateral prefrontal cortex. So, it is likely that anti-HES5 Monoclonal antibody M05384 will work for WB with dorsolateral prefrontal cortex.
Boster Scientific Support
Answered: 2019-10-03
Question
Would M05384 anti-HES5 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
As indicated on the product datasheet, M05384 anti-HES5 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-HES5 Monoclonal antibody M05384 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The anti-HES5 Monoclonal antibody (M05384) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-20
Question
My question regarding product M05384, anti-HES5 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05384 anti-HES5 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-24
Question
Is this M05384 anti-HES5 Monoclonal antibody reactive to the isotypes of HES5?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
The immunogen of M05384 anti-HES5 Monoclonal antibody is A synthesized peptide derived from human HES5 Transcriptional repressor of genes that require a bHLH protein for their transcription. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-22
