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Product Info Summary
| SKU: | A03975 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ID4 Antibody
SKU/Catalog Number
A03975
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ID4 Antibody (Catalog # A03975). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ID4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03975)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ID4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03975 is reactive to ID4 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
16622 MW
Background of ID4
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.
Rigolet M., DNA Res. 5:309-313(1998).
Pagliuca A., Genomics 27:200-203(1995).
Mungall A.J., Nature 425:805-811(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03975 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, using ID4 antibody A03975.
Protein Target Info & Infographic
Gene/Protein Information For ID4 (Source: Uniprot.org, NCBI)
Gene Name
ID4
Full Name
DNA-binding protein inhibitor ID-4
Weight
16622 MW
Alternative Names
DNA-binding protein inhibitor ID-4;Class B basic helix-loop-helix protein 27;bHLHb27;Inhibitor of DNA binding 4;Inhibitor of differentiation 4;ID4;BHLHB27; ID4 IDB4, bHLHb27 inhibitor of DNA binding 4, HLH protein DNA-binding protein inhibitor ID-4|class B basic helix-loop-helix protein 27|inhibitor of DNA binding 4, dominant negative helix-loop-helix protein|inhibitor of differentiation 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ID4, check out the ID4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ID4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ID4 Antibody (A03975)
Hello CJ!
A03975 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells
PAX7 promotes CD49f‐positive dairy goat spermatogonial stem cells self‐renewal
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-ID4 Antibody
Question
See attached the WB image, lot number and protocol we used for abdominal adipose tissue using anti-ID4 antibody A03975. Please let me know if you require anything else.
T. Edwards
Verified customer
Asked: 2018-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-27
Question
We are currently using anti-ID4 antibody A03975 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
The anti-ID4 antibody (A03975) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-20
Question
Does A03975 anti-ID4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
It shows on the product datasheet, A03975 anti-ID4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-21
Question
My question regarding product A03975, anti-ID4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03975 anti-ID4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-07
Question
I see that the anti-ID4 antibody A03975 works with WB, what is the protocol used to produce the result images on the product page?
K. Roberts
Verified customer
Asked: 2013-03-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-03-01
