Anti-Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: OTI3F11]

Product Name

Anti-Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: OTI3F11]

View all ErbB2/Her2 Antibodies

SKU/Catalog Number

M00010-4

Size

100 µl

Description

Boster Bio ERBB2 mouse monoclonal antibody, clone OTI3F11 (formerly 3F11). Catalog# M00010-4. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: OTI3F11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-4)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI3F11

Isotype

IgG2b

Immunogen

Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2 (NP_004439) produced in HEK293T cell.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00010-4 is reactive to ERBB2 in Human, Mouse, Rat

Applications

M00010-4 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2.

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Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ERBB2 monoclonal antibody (1:500).

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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HEK293T cells transfected with either ERBB2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERBB2 antibody (M00010-4)

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Flow cytometric Analysis of Jurkat cells

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Anti-ERBB2 mouse monoclonal antibody (M00010-4) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2.

Gene/Protein Information For ERBB2 (Source: Uniprot.org, NCBI)

Alternative Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomade ERBB2 CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1 erb-b2 receptor tyrosine kinase 2 receptor tyrosine-protein kinase erbB-2|c-erb B2/neu protein|herstatin|human epidermal growth factor receptor 2|metastatic lymph node gene 19 protein|neuro/glioblastoma derived oncogene homolog|neuroblastoma/glioblastoma derived oncogene homolog|proto-oncogene Neu|proto-oncogene c-ErbB-2|tyrosine kinase-type cell surface receptor HER2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2|v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2|v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

For more info on ERBB2, check out the ERBB2 Infographic

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In this infographic, you will see the following information for ERBB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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