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Product Info Summary
| SKU: | M00010-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ErbB2 (HER2) Monoclonal Antibody
View all ErbB2/Her2 Antibodies
SKU/Catalog Number
M00010-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ErbB2 (HER2) Monoclonal Antibody catalog # M00010-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ErbB2 (HER2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BCI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ErbB2 (HER2).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00010-1 is reactive to ERBB2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00010-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: Rat stomach tissue, human breast carcinoma tissue, Human esophageal carcinoma tissue
ICC/IF: SKBR cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of ErbB2 (HER2) expression in SKBR-3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ErbB2 (HER2) Antibody.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ERBB2 (Source: Uniprot.org, NCBI)
Gene Name
ERBB2
Full Name
Receptor tyrosine-protein kinase erbB-2
Weight
88068 MW
Superfamily
protein kinase superfamily
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; ERBB2 CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1 erb-b2 receptor tyrosine kinase 2 receptor tyrosine-protein kinase erbB-2|c-erb B2/neu protein|herstatin|human epidermal growth factor receptor 2|metastatic lymph node gene 19 protein|neuro/glioblastoma derived oncogene homolog|neuroblastoma/glioblastoma derived oncogene homolog|proto-oncogene Neu|proto-oncogene c-ErbB-2|tyrosine kinase-type cell surface receptor HER2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2|v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2|v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB2, check out the ERBB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ErbB2 (HER2) Monoclonal Antibody (M00010-1)
Hello CJ!
M00010-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism
Tong ZJ, Shi NY, Zhang ZJ, Yuan XD, Hong XM. Biosci Rep. 2017 Aug 2;37(4). pii: BSR20170121. doi: 10.1042/BSR20170121. Print 2017 Aug 31. Expression and prognostic value of HER-2/neu in primary breast cancer with sentinel lymph node metastasis
Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-ErbB2 (HER2) Monoclonal Antibody
Question
I see that the anti-ErbB2 (HER2) Monoclonal antibody M00010-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-26
Question
Will anti-ErbB2 (HER2) Monoclonal antibody M00010-1 work for IF with liver?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
According to the expression profile of liver, ERBB2 is highly expressed in liver. So, it is likely that anti-ErbB2 (HER2) Monoclonal antibody M00010-1 will work for IF with liver.
Boster Scientific Support
Answered: 2020-01-07
Question
I have a question about product M00010-1, anti-ErbB2 (HER2) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00010-1 anti-ErbB2 (HER2) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
Do you have a BSA free version of anti-ErbB2 (HER2) Monoclonal antibody M00010-1 available?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ErbB2 (HER2) Monoclonal antibody M00010-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-24
Question
We have seen staining in human liver. Are there any suggestions? Is anti-ErbB2 (HER2) Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
From what I have seen in literature liver does express ERBB2. From what I have seen in Uniprot.org, ERBB2 is expressed in esophagus mucosa, brain, fetal brain, mammary carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089
Boster Scientific Support
Answered: 2019-12-16
Question
We need to test anti-ErbB2 (HER2) Monoclonal antibody M00010-1 on human liver for research purposes, then I may be interested in using anti-ErbB2 (HER2) Monoclonal antibody M00010-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The products we sell, including anti-ErbB2 (HER2) Monoclonal antibody M00010-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-27
Question
I have attached the WB image, lot number and protocol we used for liver using anti-ErbB2 (HER2) Monoclonal antibody M00010-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
I am interested in using your anti-ErbB2 (HER2) Monoclonal antibody for raf/map kinase cascade studies. Has this antibody been tested with western blotting on skbr cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We appreciate your inquiry. This M00010-1 anti-ErbB2 (HER2) Monoclonal antibody is validated on skbr cells. It is guaranteed to work for IP, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-24
Question
Is a blocking peptide available for product anti-ErbB2 (HER2) Monoclonal antibody (M00010-1)?
R. Moore
Verified customer
Asked: 2019-05-07
Answer
We do provide the blocking peptide for product anti-ErbB2 (HER2) Monoclonal antibody (M00010-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-07
Question
My boss were well pleased with the WB result of your anti-ErbB2 (HER2) Monoclonal antibody. However we have seen positive staining in esophagus mucosa isoform 3: cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is ERBB2 supposed to be expressed?
V. Zhao
Verified customer
Asked: 2018-12-03
Answer
From what I have seen in literature, esophagus mucosa does express ERBB2. Generally ERBB2 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. nucleus., isoform 3: cytoplasm. nucleus. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089
Boster Scientific Support
Answered: 2018-12-03
Question
Does M00010-1 anti-ErbB2 (HER2) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Parker
Verified customer
Asked: 2018-03-07
Answer
It shows on the product datasheet, M00010-1 anti-ErbB2 (HER2) Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-07
Question
I was wanting to use your anti-ErbB2 (HER2) Monoclonal antibody for IF for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
As indicated on the product datasheet, M00010-1 anti-ErbB2 (HER2) Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-24
Question
We have tried in the past anti-ErbB2 (HER2) Monoclonal antibody for IHC on cervix carcinoma erythroleukemia a few months ago. I am using human, and We intend to use the antibody for IP next. I am interested in examining cervix carcinoma erythroleukemia as well as esophagus mucosa in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
I looked at the website and datasheets of our anti-ErbB2 (HER2) Monoclonal antibody and I see that M00010-1 has been tested on human in both IHC and IP. Thus M00010-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-06
Question
Is this M00010-1 anti-ErbB2 (HER2) Monoclonal antibody reactive to the isotypes of ERBB2?
B. Wu
Verified customer
Asked: 2017-11-03
Answer
The immunogen of M00010-1 anti-ErbB2 (HER2) Monoclonal antibody is A synthesized peptide derived from human ErbB2 (HER2). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-03
Question
We are currently using anti-ErbB2 (HER2) Monoclonal antibody M00010-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
B. Yang
Verified customer
Asked: 2015-09-15
Answer
The anti-ErbB2 (HER2) Monoclonal antibody (M00010-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ErbB2 (HER2) Monoclonal antibody M00010-1. Let me know if you need anything else.
P. Rodriguez
Verified customer
Asked: 2015-07-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-06
