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Product Info Summary
| SKU: | A04607 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Heparin Cofactor II Antibody
View all Serpin D1/Heparin Cofactor II Antibodies
SKU/Catalog Number
A04607
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Heparin Cofactor II Antibody (Catalog # A04607). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heparin Cofactor II Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04607)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Heparin Cofactor II.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04607 is reactive to SERPIND1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
57071 MW
Background of Serpin D1/Heparin Cofactor II
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.
Blinder M.A., Biochemistry 27:752-759(1988).
Herzog R., Biochemistry 30:1350-1357(1991).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04607 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB dilution: 1:500-1:3000
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using Heparin Cofactor II antibody A04607.
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Immunofluorescence analysis of HepG2 cells, using Heparin Cofactor II antibody A04607.
Protein Target Info & Infographic
Gene/Protein Information For SERPIND1 (Source: Uniprot.org, NCBI)
Gene Name
SERPIND1
Full Name
Heparin cofactor 2
Weight
57071 MW
Superfamily
serpin family
Alternative Names
Heparin cofactor 2;Heparin cofactor II;HC-II;Protease inhibitor leuserpin-2;HLS2;Serpin D1;SERPIND1;HCF2; SERPIND1 D22S673, HC2, HCF2, HCII, HLS2, LS2, THPH10 serpin family D member 1 heparin cofactor 2|heparin cofactor II splice isoform|leuserpin 2|protease inhibitor leuserpin-2|serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1|serpin D1|serpin peptidase inhibitor, clade D (heparin cofactor), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPIND1, check out the SERPIND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPIND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heparin Cofactor II Antibody (A04607)
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No publications found for A04607
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Heparin Cofactor II Antibody
Question
I was wanting to use to test anti-Heparin Cofactor II antibody A04607 on human liver for research purposes, then I may be interested in using anti-Heparin Cofactor II antibody A04607 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The products we sell, including anti-Heparin Cofactor II antibody A04607, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-20
Question
Will A04607 anti-Heparin Cofactor II antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
As indicated on the product datasheet, A04607 anti-Heparin Cofactor II antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
Will anti-Heparin Cofactor II antibody A04607 work on dog IF with plasma?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Our lab technicians have not tested anti-Heparin Cofactor II antibody A04607 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Heparin Cofactor II antibody A04607 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-22
Question
I was wanting to use your anti-Heparin Cofactor II antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
It shows on the product datasheet, A04607 anti-Heparin Cofactor II antibody has been tested for IF, IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Heparin Cofactor II antibody A04607. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-Heparin Cofactor II antibody A04607 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
The anti-Heparin Cofactor II antibody (A04607) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-10
Question
Is there a BSA free version of anti-Heparin Cofactor II antibody A04607 available?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Heparin Cofactor II antibody A04607 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-02
Question
I have a question about product A04607, anti-Heparin Cofactor II antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04607 anti-Heparin Cofactor II antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-26
Question
I see that the anti-Heparin Cofactor II antibody A04607 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-02
Question
Is this A04607 anti-Heparin Cofactor II antibody reactive to the isotypes of SERPIND1?
C. Krishna
Verified customer
Asked: 2017-12-05
Answer
The immunogen of A04607 anti-Heparin Cofactor II antibody is Synthesized peptide derived from internal of human Heparin Cofactor II. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-05
Question
Please see the WB image, lot number and protocol we used for liver using anti-Heparin Cofactor II antibody A04607. Please let me know if you require anything else.
C. Dhar
Verified customer
Asked: 2015-11-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-30
Question
Is a blocking peptide available for product anti-Heparin Cofactor II antibody (A04607)?
S. Anderson
Verified customer
Asked: 2014-03-18
Answer
We do provide the blocking peptide for product anti-Heparin Cofactor II antibody (A04607). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-18
Question
Would anti-Heparin Cofactor II antibody A04607 work for IHC with liver?
S. Jones
Verified customer
Asked: 2013-07-15
Answer
According to the expression profile of liver, SERPIND1 is highly expressed in liver. So, it is likely that anti-Heparin Cofactor II antibody A04607 will work for IHC with liver.
Boster Scientific Support
Answered: 2013-07-15
