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Product Info Summary
| SKU: | M02029 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cow, Human, Monkey, Mouse, Pig, Fish |
| Host: | Rat |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody
SKU/Catalog Number
M02029
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody (Catalog # M02029). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Cow, Pig, Mouse, Fish.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02029)
Host
Rat
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM255
Isotype
IgG2a, kappa
Immunogen
Murine EHS laminin preparation
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02029 is reactive to HSPG2 in Cow, Human, Monkey, Mouse, Pig, Fish
Reconstitution
Observed Molecular Weight
19 kDa
Calculated molecular weight
468830 MW
Background of HSPG2
This monoclonal antibody specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02029 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-Heparan Sulfate Monoclonal Antibody (SPM255).
Protein Target Info & Infographic
Gene/Protein Information For HSPG2 (Source: Uniprot.org, NCBI)
Gene Name
HSPG2
Full Name
Basement membrane-specific heparan sulfate proteoglycan core protein
Weight
468830 MW
Alternative Names
Basement membrane-specific heparan sulfate proteoglycan core protein;HSPG;Perlecan;PLC;Endorepellin;LG3 peptide;HSPG2; HSPG2 HSPG, PLC, PRCAN, SJA, SJS, SJS1 heparan sulfate proteoglycan 2 basement membrane-specific heparan sulfate proteoglycan core protein|endorepellin (domain V region)|perlecan proteoglycan
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPG2, check out the HSPG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody (M02029)
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No publications found for M02029
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Customer Q&As
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7 Customer Q&As for Anti-Heparan Sulfate Proteoglycan (Large) / Perlecan HSPG2 Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for colon using anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-08
Question
Would M02029 anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
You can see on the product datasheet, M02029 anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-12
Question
Will anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 work on zebrafish IHC with liver?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Our lab technicians have not validated anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use your anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody for IHC for monkey colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for monkey colon identification?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
As indicated on the product datasheet, M02029 anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody has been validated for IHC on bovine, human, monkey, mouse, pig tissues. We have an innovator award program that if you test this antibody and show it works in monkey colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-09
Question
I have a question about product M02029, anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02029 anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-03
Question
I am interested in to test anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 on monkey colon for research purposes, then I may be interested in using anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Krishna
Verified customer
Asked: 2017-08-18
Answer
The products we sell, including anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-18
Question
Do you have a BSA free version of anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 available?
K. Huang
Verified customer
Asked: 2014-03-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Heparan Sulfate Proteoglycan (Large) / Perlecan Monoclonal antibody M02029 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-28
