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Product Info Summary
| SKU: | EK1826 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Plexin B2/PLXNB2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1826
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Plexin B2/PLXNB2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1826)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L20-L1838
Sensitivity
<0.1 ng/ml
Assay Range
0.624 ng/ml - 40 ng/ml
Standard Dilution Instructions
See datasheet of EK1826 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1826 is reactive to PLXNB2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1826 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Plexin B2
Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1826-CAP | Anti-Human PLXNB2 Pre-coated 96-well strip microplate | 1 |
| EK1826-ST | Human PLXNB2 Standard | 2 vials, 40 ng/tube |
| EK1826-DA | Human PLXNB2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.63 | 1.25 | 2.5 | 5 | 10 | 20 | 40 |
| O.D. | 0.029 | 0.150 | 0.299 | 0.381 | 0.731 | 0.929 | 1.346 | 1.963 |
Data Example Images
Click image to see more details
Human Plexin B2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Plexin B2, Dilution ratio of 1:4, concentration in serum and plasma is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Plexin B2/PLXNB2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1214 | 4275 | 19603 | 1233 | 4407 | 19741 |
| Standard deviation | 63.13 | 239.4 | 960.55 | 82.6 | 339.3 | 1539.8 |
| CV (%) | 5.2% | 5.6% | 4.9% | 6.7% | 7.7% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Plexin B2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1214 | 1018 | 1165 | 1017 | 1103 | 87.72 | 7.9% |
| Sample 2 | 4275 | 4570 | 4511 | 4741 | 4524 | 166.86 | 3.6% |
| Sample 3 | 19603 | 18628 | 19571 | 18283 | 19021 | 578.86 | 3.0% |
Protein Target Info & Infographic
Gene/Protein Information For PLXNB2 (Source: Uniprot.Org, NCBI)
Gene Name
PLXNB2
Full Name
Plexin-B2
Weight
205.127kDa
Superfamily
plexin family
Alternative Names
Plexin-B2; MM1; PLXNB2; KIAA0315 PLXNB2 MM1, Nbla00445, PLEXB2, dJ402G11.3 plexin B2 plexin-B2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PLXNB2, check out the PLXNB2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLXNB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Plexin B2/PLXNB2 ELISA Kit PicoKine® (EK1826)
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No publications found for EK1826
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
5 Customer Q&As for Human Plexin B2/PLXNB2 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have performed Plexin B2 level in plasma.
Z. Dayal
Verified customer
Asked: 2020-05-13
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-13
Question
Q: if the enzyme conjugated Plexin B2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Plexin B2 antigen?
M. Chakrabarti
Verified customer
Asked: 2020-01-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-01-04
Question
Q: Can Plexin B2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Singh
Verified customer
Asked: 2019-06-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-06-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-09-11
Question
Q: how to thaw whole blood sample for Plexin B2 ELISA after freezing?
L. Jackson
Verified customer
Asked: 2014-05-26
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Plexin B2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2014-05-26
