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Product Info Summary
| SKU: | A30472 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-HEN1/2 NHLH1 Antibody
SKU/Catalog Number
A30472
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HEN1/2 NHLH1 Antibody (Catalog# A30472). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEN1/2 NHLH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30472)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HEN1/2. Immunogen sequence location: 41-90
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30472 is reactive to NHLH1 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
14618 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30472 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using HEN1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For NHLH1 (Source: Uniprot.org, NCBI)
Gene Name
NHLH1
Full Name
Helix-loop-helix protein 1
Weight
14618 MW
Alternative Names
NHLH1;BHLHA35;HEN1;Helix-loop-helix protein 1;HEN-1;Class A basic helix-loop-helix protein 35;bHLHa35;Nescient helix loop helix 1;NSCL-1;NHLH2;BHLHA34;HEN2;KIAA0490;Helix-loop-helix protein 2;HEN-2;Class A basic helix-loop-he NHLH1 HEN1, NSCL, NSCL1, bHLHa35 nescient helix-loop-helix 1 helix-loop-helix protein 1|HEN-1|NSCL-1|class A basic helix-loop-helix protein 35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NHLH1, check out the NHLH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NHLH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HEN1/2 NHLH1 Antibody (A30472)
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No publications found for A30472
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-HEN1/2 NHLH1 Antibody
Question
Is there a BSA free version of anti-HEN1/2 antibody A30472 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HEN1/2 antibody A30472 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
Will anti-HEN1/2 antibody A30472 work for WB with eye?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
According to the expression profile of eye, NHLH1 is highly expressed in eye. So, it is likely that anti-HEN1/2 antibody A30472 will work for WB with eye.
Boster Scientific Support
Answered: 2019-09-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-HEN1/2 antibody A30472. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-HEN1/2 antibody A30472 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The anti-HEN1/2 antibody (A30472) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
Question
I would like to test anti-HEN1/2 antibody A30472 on human eye for research purposes, then I may be interested in using anti-HEN1/2 antibody A30472 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-11
Answer
The products we sell, including anti-HEN1/2 antibody A30472, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-11
