Product Info Summary
SKU: | A02237-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hemopexin HPX Antibody
SKU/Catalog Number
A02237-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Hemopexin HPX Antibody catalog # A02237-1. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hemopexin HPX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02237-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HCN4 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02237-1 is reactive to HPX in Human
Applications
A02237-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
51676 MW
Background of Hemopexin
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Lawrance S.K., Nucleic Acids Res. 13:7515-7528(1985).
Gustafsson K., J. Biol. Chem. 262:8778-8786(1987).
Young J.A., Hum. Immunol. 23:37-44(1988).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:100-1:500
IP: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of HPX using Anti-Hemopexin HPX Antibody (A02237-1).
ICC staining Hemopexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Hemopexin HPX Antibody A02237-1
Western blot analysis of Hemopexin on human plasma lysate using anti-Hemopexin antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For HPX (Source: Uniprot.org, NCBI)
Gene Name
HPX
Full Name
Hemopexin
Weight
51676 MW
Superfamily
hemopexin family
Alternative Names
Beta-1B-glycoprotein; FLJ56652; Hemopexin; HPX; HX HPX HX hemopexin hemopexin|beta-1B-glycoprotein|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HPX, check out the HPX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HPX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hemopexin HPX Antibody (A02237-1)
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