Anti-HDAC2 Antibody Picoband®

Histone Deacetylase 2/HDAC2 antibody

Boster Bio Anti-HDAC2 Antibody Picoband® catalog # A00325-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 2 publication(s).

Product Info Summary

SKU: A00325-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-HDAC2 Antibody Picoband®

View all Histone Deacetylase 2/HDAC2 Antibodies

SKU/Catalog Number

A00325-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HDAC2 Antibody Picoband® catalog # A00325-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HDAC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00325-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HDAC2 recombinant protein (Position: E387-P488).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00325-3 is reactive to HDAC2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

60 kDa

Calculated molecular weight

29041 MW

Background of Histone Deacetylase 2/HDAC2

Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al. (1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00325-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human K562 whole cell, human THP-1 whole cell, human HL-60 whole cell, human Caco-2 whole cell, human HepG2 whole cell, rat brain tissue, rat lung tissue, rat pancreas tissue, mouse brain tissue, mouse lung tissue
IHC: human breast cancer tissue, human cervical cancer tissue, human endometrial cancer tissue, human gallbladder adenocarcinoma tissue, human gastric cancer tissue, human lung cancer tissue, human ovarian cancer tissue, mouse testis tissue
ICC/IF: Caco-2 cell
FCM: HL-60 cell

Validation Images & Assay Conditions

Gene/Protein Information For HDAC2 (Source: Uniprot.org, NCBI)

Gene Name

HDAC2

Full Name

Histone deacetylase 2

Weight

29041 MW

Superfamily

histone deacetylase family

Alternative Names

Bone morphogenetic protein receptor type-2; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; BMP type II receptor; BMPR-II; BMPR2; PPH1 HDAC2 HD2, KDAC2, RPD3, YAF1 histone deacetylase 2 histone deacetylase 2|YY1-associated factor 1|transcriptional regulator homolog RPD3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HDAC2, check out the HDAC2 Infographic

HDAC2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HDAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00325-3 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of GR-α and HDAC2 in steroid-Sensitive and steroid-Insensitive interstitial lung disease

Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...

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Order DetailsPrice
A00325-3

100μg

$370
A00325-3-10ug

10μg sample (liquid)

$99
A00325-3-Biotin

100 μg Biotin conjugated

$570
A00325-3-Cy3

100 μg Cy3 conjugated

$570
A00325-3-Dylight488

100 μg Dylight488 conjugated

$570
A00325-3-Dylight550

100 μg Dylight550 conjugated

$570
A00325-3-Dylight594

100 μg Dylight594 conjugated

$570
A00325-3-FITC

100 μg FITC conjugated

$570
A00325-3-HRP

100 μg HRP conjugated

$570
A00325-3-APC

100 μg APC conjugated

$670
A00325-3-PE

100 μg PE conjugated

$670
A00325-3-iFluor647

100 μg iFluor647 conjugated

$670
A00325-3-carrier-free

Carrier Free

$370

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A00325-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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