Product Info Summary
SKU: | A00256-1 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-HDAC-1 (C-terminus) Antibody
SKU/Catalog Number
A00256-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HDAC-1 (C-terminus) Antibody (Catalog # A00256-1). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-HDAC-1 (C-terminus) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00256-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00256-1 is reactive to HDAC1 in Human
Reconstitution
Calculated molecular weight
55103 MW
Background of HDAC1
HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00256-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: 1:200 - 1:1,000
IF Microscopy: 10ug / ml
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
Click image to see more details
HDAC-1 was detected in paraffin-embedded sections of human prostate cancer tissue using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 1:500. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
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Western blot analysis of HDAC-1 expression in 293 whole cell lysate. HDAC-1 at 65KD was detected using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1)at 1:3500.
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HDAC-1 was detected in paraffin-embedded sections of human lung tissue using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
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Immunofluorescence Microscopy of Rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10
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HDAC-1 was detected in immunocytochemical sections of A431 cells using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 0.4
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Western blot analysis of HDAC-1 expression in LNCaP prostate cancer cells. HDAC-1 at 55KD was detected using rabbit anti-HDAC-1 Antigen Affinity purified polyclonal antibody (Catalog # A00256-1) at 1:1000.
Protein Target Info & Infographic
Gene/Protein Information For HDAC1 (Source: Uniprot.org, NCBI)
Gene Name
HDAC1
Full Name
Histone deacetylase 1
Weight
55103 MW
Superfamily
histone deacetylase family
Alternative Names
HDAC-1, HDAC1, HDAC 1, HD 1, histone deacetylase 1, Histone deacetylase-1, HD1, RPD3L1, reduced potassium dependency yeast homolog like 1 HDAC1 GON-10, HD1, KDAC1, RPD3, RPD3L1 histone deacetylase 1 histone deacetylase 1|reduced potassium dependency, yeast homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC1, check out the HDAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC-1 (C-terminus) Antibody (A00256-1)
Hello CJ!
A00256-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification of candidate biomarkers that involved in the epigenetic transcriptional regulation for detection gastric cancer by iTRAQ based quantitative proteomic analysis
Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...
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