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Product Info Summary
| SKU: | PA1552 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-hCG receptor/LHCGR Antibody Picoband®
SKU/Catalog Number
PA1552
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-hCG receptor/LHCGR Antibody catalog # PA1552. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hCG receptor/LHCGR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1552)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human hCG receptor, different from the relative rat sequence by two amino acids, mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1552 is reactive to LHCGR in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
78643 MW
Background of LHR
The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors (GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats (LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR (LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage (luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1552 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human , By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: MCF-7 Whole Cell
IHC: Rat Ovary Tissue
Validation Images & Assay Conditions
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Anti-hCG receptor antibody, PA1552, IHC(P)
IHC(P): Rat Ovary Tissue
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Anti-hCG receptor antibody, PA1552, Western blotting
All lanes: Anti hCG receptor (PA1552) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
Protein Target Info & Infographic
Gene/Protein Information For LHCGR (Source: Uniprot.org, NCBI)
Gene Name
LHCGR
Full Name
Lutropin-choriogonadotropic hormone receptor
Weight
78643 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Lutropin-choriogonadotropic hormone receptor;LH/CG-R;Luteinizing hormone receptor;LHR;LSH-R;LHCGR;LCGR, LGR2, LHRHR; LHCGR HHG, LCGR, LGR2, LH/CG-R, LH/CGR, LHR, LHRHR, LSH-R, ULG5 luteinizing hormone/choriogonadotropin receptor lutropin-choriogonadotropic hormone receptor|hypergonadotropic hypogonadism|lutropin/choriogonadotropin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LHCGR, check out the LHCGR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LHCGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hCG receptor/LHCGR Antibody Picoband® (PA1552)
Hello CJ!
PA1552 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Evaluation of Follicular Synchronization Caused by Estrogen Administration and Its Reproductive Outcome
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Customer Q&As
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2 Customer Q&As for Anti-hCG receptor/LHCGR Antibody Picoband®
Question
What are your suggestions to reduce the background for my western blot? Keyword: troubleshooting, protocol support
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Our technicians provide these suggestions to reduce the background: 1.Block the membrane 2 hours at room temperature with 5% no-fat milk in TBS-T; 2.Dilute second antibody with 5% no-fat milk in TBS-T; and 3.Extend the wash time to 10 minutes each time.
Boster Scientific Support
Answered: 2019-10-21
Question
We are currently using anti-hCG receptor/LHCGR antibody PA1552 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The anti-hCG receptor/LHCGR antibody (PA1552) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-20
