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Product Info Summary
| SKU: | A00268S612 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IRS-1 (phospho-S612) Antibody
SKU/Catalog Number
A00268S612
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IRS-1 (phospho-S612) Antibody catalog # A00268S612. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRS-1 (phospho-S612) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00268S612)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Tyrosine 612.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00268S612 is reactive to IRS1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
131591 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00268S612 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of IRS1 using Anti-IRS-1 (phospho-S612) Antibody (A00268S612).
Immunohistochemistry (IHC) analyzes of P-IRS-1 (S612) pAbin paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-IRS-1 (phospho-S612) Antibody A00268S612
Western blot (WB) analysis of p-IRS-1 (S612) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:A375 whole cell lysate(40ug)
Lane4:A2780 whole cell lysate(40ug)
Lane5:Hela whole cell lysate(40ug)
Lane6:C6 whole cell lysate(40ug)
Lane7:NIH-3T3 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For IRS1 (Source: Uniprot.org, NCBI)
Gene Name
IRS1
Full Name
Insulin receptor substrate 1
Weight
131591 MW
Alternative Names
Insulin receptor substrate 1;IRS-1;IRS1; IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRS1, check out the IRS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRS-1 (phospho-S612) Antibody (A00268S612)
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No publications found for A00268S612
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