Anti-HCFC1/Hcf1 Antibody

Product Name

Anti-HCFC1/Hcf1 Antibody

View all Host Cell Factor 1/HCFC1 Antibodies

SKU/Catalog Number

A01729

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-HCFC1/Hcf1 Antibody (Catalog # A01729). Tested in IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HCFC1/Hcf1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01729)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human HCFC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01729 is reactive to HCFC1 in Human, Mouse

Reconstitution

Calculated molecular weight

208732 MW

Background of Host Cell Factor 1/HCFC1

Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.

Wilson A.C., Cell 74:115-125(1993).
Kristie T.M., J. Biol. Chem. 270:4387-4394(1995).
Ross M.T., Nature 434:325-337(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01729 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For HCFC1 (Source: Uniprot.org, NCBI)

Alternative Names

Host cell factor 1;HCF;HCF-1;C1 factor;CFF;VCAF;VP16 accessory protein;HCF N-terminal chain 1;HCF N-terminal chain 2;HCF N-terminal chain 3;HCF N-terminal chain 4;HCF N-terminal chain 5;HCF N-terminal chain 6;HCF C-terminal chain 1;HCF C-terminal chain 2;HCF C-terminal chain 3;HCF C-terminal chain 4;HCF C-terminal chain 5;HCF C-terminal chain 6;HCFC1;HCF1, HFC1; HCFC1 CFF, HCF, HCF-1, HCF1, HFC1, MRX3, PPP1R89, VCAF host cell factor C1 host cell factor 1|VP16-accessory protein|protein phosphatase 1, regulatory subunit 89

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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