Product Info Summary
SKU: | A03596 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HAT HAT1 Antibody
SKU/Catalog Number
A03596
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HAT HAT1 Antibody (Catalog # A03596). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HAT HAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03596)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human HAT.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03596 is reactive to HAT1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
49513 MW
Background of KAT1
Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Claudia J. Schroder-Adams, Bulletin of Canadian Petroleum Geology, Sep 1997; 45: 356 - 376.
Pil-Hoon Park, Am J Physiol Gastrointest Liver Physiol, Dec 2005; 289: G1124 - G1136.
Claudia J. Schroeder-Adams, Palaios, Aug 1998; 13: 361 - 375.
L. J. Patric
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03596 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using HAT antibody A03596.
Protein Target Info & Infographic
Gene/Protein Information For HAT1 (Source: Uniprot.org, NCBI)
Gene Name
HAT1
Full Name
Histone acetyltransferase type B catalytic subunit
Weight
49513 MW
Superfamily
HAT1 family
Alternative Names
Histone acetyltransferase type B catalytic subunit;2.3.1.48 ;Histone acetyltransferase 1;HAT1;KAT1; HAT1 KAT1 histone acetyltransferase 1 histone acetyltransferase type B catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HAT1, check out the HAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HAT HAT1 Antibody (A03596)
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No publications found for A03596
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Customer Q&As
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3 Customer Q&As for Anti-HAT HAT1 Antibody
Question
I see that the anti-HAT antibody A03596 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-HAT antibody A03596 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-HAT antibody (A03596) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
I have a question about product A03596, anti-HAT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Krishna
Verified customer
Asked: 2019-10-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03596 anti-HAT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-24