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Product Info Summary
| SKU: | M01759-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HB EGF Rabbit Monoclonal Antibody
SKU/Catalog Number
M01759-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-HB EGF Rabbit Monoclonal Antibody catalog # M01759-1. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HB EGF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01759-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
21H08
Isotype
IgG
Immunogen
A synthesized peptide derived from human HB EGF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01759-1 is reactive to HBEGF in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
23 kDa
Calculated molecular weight
72692 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01759-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human K562 whole cell, human Hela whole cell, human Jurkat whole cell, human U251 whole cell, rat lung tissue, rat C6 whole cell, mouse lung tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HBEGF using anti-HBEGF antibody (M01759-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBEGF antigen affinity purified monoclonal antibody (Catalog # M01759-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HBEGF at approximately 23 kDa. The expected band size for HBEGF is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HBEGF (Source: Uniprot.org, NCBI)
Gene Name
HBEGF
Full Name
Proheparin-binding EGF-like growth factor
Weight
72692 MW
Alternative Names
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial;1.3.5.1;Flavoprotein subunit of complex II;Fp;SDHA;SDH2, SDHF; HBEGF DTR, DTS, DTSF, HEGFL heparin binding EGF like growth factor proheparin-binding EGF-like growth factor|diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|heparin-binding epidermal growth factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HBEGF, check out the HBEGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HBEGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HB EGF Rabbit Monoclonal Antibody (M01759-1)
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