Product Info Summary
SKU: | A13030 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-ES8L3 Antibody
SKU/Catalog Number
A13030
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ES8L3 Antibody (Catalog # A13030). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ES8L3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13030)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ES8L3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13030 is reactive to EPS8L3 in Human
Reconstitution
Calculated molecular weight
66861 MW
Background of EPS8L3
This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. Alternatively spliced transcript variants encoding different isoforms exist.
Tocchetti A., Genomics 81:234-244(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13030 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ES8L3 antibody A13030.
Click image to see more details
Western blot analysis of extracts from Cos7 cells and K562 cells, using ES8L3 antiobdy A13030.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using ES8L3 antibody A13030.
Protein Target Info & Infographic
Gene/Protein Information For EPS8L3 (Source: Uniprot.org, NCBI)
Gene Name
EPS8L3
Full Name
Epidermal growth factor receptor kinase substrate 8-like protein 3
Weight
66861 MW
Superfamily
EPS8 family
Alternative Names
Epidermal growth factor receptor kinase substrate 8-like protein 3;EPS8-like protein 3;Epidermal growth factor receptor pathway substrate 8-related protein 3;EPS8-related protein 3;EPS8L3;EPS8R3; EPS8L3 EPS8R3, HYPT5 EPS8 like 3 epidermal growth factor receptor kinase substrate 8-like protein 3|EPS8-like protein 3|EPS8-related protein 3|epidermal growth factor receptor pathway substrate 8-related protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPS8L3, check out the EPS8L3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPS8L3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ES8L3 Antibody (A13030)
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No publications found for A13030
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-ES8L3 Antibody
Question
I see that the anti-ES8L3 antibody A13030 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-27
Question
Is a blocking peptide available for product anti-ES8L3 antibody (A13030)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-ES8L3 antibody (A13030). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
We are currently using anti-ES8L3 antibody A13030 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
The anti-ES8L3 antibody (A13030) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-30
Question
See attached the WB image, lot number and protocol we used for transverse colon using anti-ES8L3 antibody A13030. Please let me know if you require anything else.
T. Jha
Verified customer
Asked: 2018-08-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-31
Question
Does anti-ES8L3 antibody A13030 work on canine IHC with cervix carcinoma?
B. Jones
Verified customer
Asked: 2017-06-16
Answer
Our lab technicians have not tested anti-ES8L3 antibody A13030 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ES8L3 antibody A13030 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16