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Product Info Summary
| SKU: | PA1040 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GST3/GST pi/GSTP1 Antibody Picoband®
View all Glutathione S-Transferase pi 1/GSTP1 Antibodies
SKU/Catalog Number
PA1040
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GST3/GST pi/GSTP1 Antibody catalog # PA1040. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GST3/GST pi/GSTP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1040)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GST3, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1040 is reactive to GSTP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23356 MW
Background of Glutathione S-Transferase pi 1/GSTP1
Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1040 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat lung tissue, mouse lung tissue, mouse liver tissue
IHC: human highly differentiated adenocarcinoma of the colon tissue, human moderately differentiated adenocarcinoma of the cervical canal tissue, human ovarian cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GSTP1 using anti-GSTP1 antibody (PA1040).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTP1 antigen affinity purified polyclonal antibody (Catalog # PA1040) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTP1 at approximately 23 kDa. The expected band size for GSTP1 is at 23 kDa.
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Figure 2. IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040).
GSTP1 was detected in a paraffin-embedded section of human highly differentiated adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040).
GSTP1 was detected in a paraffin-embedded section of human moderately differentiated adenocarcinoma of the cervical canal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040).
GSTP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GSTP1 (Source: Uniprot.org, NCBI)
Gene Name
GSTP1
Full Name
Glutathione S-transferase P
Weight
23356 MW
Superfamily
GST superfamily
Alternative Names
Glutathione S-transferase P;2.5.1.18;GST class-pi;GSTP1-1;GSTP1;FAEES3, GST3; GSTP1 DFN7, FAEES3, GST3, GSTP, HEL-S-22, PI glutathione S-transferase pi 1 glutathione S-transferase P|GST class-pi|GSTP1-1|deafness, X-linked 7|epididymis secretory protein Li 22|fatty acid ethyl ester synthase III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSTP1, check out the GSTP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSTP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GST3/GST pi/GSTP1 Antibody Picoband® (PA1040)
Hello CJ!
PA1040 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expressions of glutathione S‐transferase alpha, mu, pi, and theta in the skin samples of patients with acne rosacea
Glutathione S-Transferases and Cytochrome P450 enzyme expression in patients with Intracranial Tumors: Preliminary Report of 55 Patients
Enhanced expression of stem cell markers and drug resistance in sphere-forming non-small cell lung cancer cells.
The recovery trajectory of adolescent social defeat stress-induced behavioral, (1)H-MRS metabolites and myelin changes in Balb/c mice
Yang P, Gao Z, Zhang H, Fang Z, Wu C, Xu H, Huang Qj. Neuropsychiatr Dis Treat. 2015 Mar 6;11:597-607. Doi: 10.2147/Ndt.S78131. Ecollection 2015. Changes In Proinflammatory Cytokines And White Matter In Chronically Stressed Rats.
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Customer Q&As
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7 Customer Q&As for Anti-GST3/GST pi/GSTP1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-GST3/GST pi/GSTP1 antibody (PA1040)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
We do provide the blocking peptide for product anti-GST3/GST pi/GSTP1 antibody (PA1040). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-GST3/GST pi/GSTP1 antibody PA1040. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-17
Question
Is this PA1040 anti-GST3/GST pi/GSTP1 antibody reactive to the isotypes of GSTP1?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The immunogen of PA1040 anti-GST3/GST pi/GSTP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human GST3(197-210aa PEYVNLPINGNGKQ), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-12
Question
I am interested in to test anti-GST3/GST pi/GSTP1 antibody PA1040 on mouse urinary bladder for research purposes, then I may be interested in using anti-GST3/GST pi/GSTP1 antibody PA1040 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The products we sell, including anti-GST3/GST pi/GSTP1 antibody PA1040, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-05
Question
Do you have a BSA free version of anti-GST3/GST pi/GSTP1 antibody PA1040 available?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GST3/GST pi/GSTP1 antibody PA1040 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-03
Question
Does anti-GST3/GST pi/GSTP1 antibody PA1040 work for WB with urinary bladder?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
According to the expression profile of urinary bladder, GSTP1 is highly expressed in urinary bladder. So, it is likely that anti-GST3/GST pi/GSTP1 antibody PA1040 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2018-03-14
Question
Will anti-GST3/GST pi/GSTP1 antibody PA1040 work on horse WB with esophagus mucosa?
M. Miller
Verified customer
Asked: 2014-05-29
Answer
Our lab technicians have not validated anti-GST3/GST pi/GSTP1 antibody PA1040 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GST3/GST pi/GSTP1 antibody PA1040 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse esophagus mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-05-29
