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Product Info Summary
| SKU: | A00791-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-GSK3b (Ab-9) Antibody
View all GSK-3 beta Antibodies
SKU/Catalog Number
A00791-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GSK3b (Ab-9) Antibody (Catalog # A00791-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GSK3b (Ab-9) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00791-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.7~11 (T-T-S-F-A) derived from Human GSK3b.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00791-1 is reactive to GSK3B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
138564 MW
Background of GSK-3 beta
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.
Cross D. A, et al. (1995) Nature. 378: 785-789.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00791-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GSK3B using anti-GSK3B antibody (A00791-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3B antigen affinity purified polyclonal antibody (Catalog # A00791-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GSK3B.
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Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic and nuclear staining using GSK3b(Ab-9) Antibody #A00791-1.
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Figure 2. IHC analysis of GSK3B using anti-GSK3B antibody (A00791-1).
GSK3B was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (A00791-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GSK3B (Source: Uniprot.org, NCBI)
Gene Name
GSK3B
Full Name
Glycogen synthase kinase-3 beta
Weight
138564 MW
Superfamily
protein kinase superfamily
Alternative Names
Collagen alpha-1 (III) chain;COL3A1; GSK3B glycogen synthase kinase 3 beta glycogen synthase kinase-3 beta|GSK-3 beta|serine/threonine-protein kinase GSK3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSK3B, check out the GSK3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSK3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GSK3b (Ab-9) Antibody (A00791-1)
Hello CJ!
A00791-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice
GSK%u20113%u03B2 may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole%u2011kindling model
Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-GSK3b (Ab-9) Antibody
Question
Would A00791-1 anti-GSK3b (Ab-9) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
It shows on the product datasheet, A00791-1 anti-GSK3b (Ab-9) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-07
Question
Is a blocking peptide available for product anti-GSK3b (Ab-9) antibody (A00791-1)?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
We do provide the blocking peptide for product anti-GSK3b (Ab-9) antibody (A00791-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-GSK3b (Ab-9) antibody A00791-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
I see that the anti-GSK3b (Ab-9) antibody A00791-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-22
Question
My question regards to test anti-GSK3b (Ab-9) antibody A00791-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-GSK3b (Ab-9) antibody A00791-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The products we sell, including anti-GSK3b (Ab-9) antibody A00791-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-29
Question
Is this A00791-1 anti-GSK3b (Ab-9) antibody reactive to the isotypes of GSK3B?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
The immunogen of A00791-1 anti-GSK3b (Ab-9) antibody is Peptide sequence around aa.7~11 (T-T-S-F-A) derived from Human GSK3b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-25
Question
Do you have a BSA free version of anti-GSK3b (Ab-9) antibody A00791-1 available?
K. Evans
Verified customer
Asked: 2013-07-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GSK3b (Ab-9) antibody A00791-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-23
