Click image to see more details
-
-
-
-
-
+7
Product Info Summary
| SKU: | M00955 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
View all GRP78/HSPA5 Antibodies
SKU/Catalog Number
M00955
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody catalog # M00955. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BOO-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GRP78 BiP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00955 is reactive to HSPA5 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
72333 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00955 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Human non-Hodgkin's lymphoma tissue, Human thyroid cancer tissue, Human prostate cancer tissue, Rat testis tissue, Mouse skin tissue, human pancreas tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of GRP78 BiP expression in (1) LnCaP cell lysate;(2) HepG2 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:200 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:200 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis of HepG2 cells, using GRP78 BiP Antibody .
Protein Target Info & Infographic
Gene/Protein Information For HSPA5 (Source: Uniprot.org, NCBI)
Gene Name
HSPA5
Full Name
Endoplasmic reticulum chaperone BiP
Weight
72333 MW
Superfamily
heat shock protein 70 family
Alternative Names
78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP;HSPA5;GRP78; HSPA5 BIP, GRP78, HEL-S-89n heat shock protein family A (Hsp70) member 5 endoplasmic reticulum chaperone BiP|78 kDa glucose-regulated protein|HSP70 family protein 5|binding-immunoglobulin protein|endoplasmic reticulum lumenal Ca(2+)-binding protein grp78|epididymis secretory sperm binding protein Li 89n|glucose-regulated protein, 78kDa|heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|heat shock protein 70 family protein 5|heat shock protein family A member 5|immunoglobulin heavy chain-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA5, check out the HSPA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (M00955)
Hello CJ!
M00955 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxicity in rats
Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
M00955 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1200
Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways
Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody
Question
I am looking for using your anti-GRP78 BiP Rabbit Monoclonal antibody for toxin transport studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
We appreciate your inquiry. This M00955 anti-GRP78 BiP Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-05
Question
We have observed staining in human brain. Any tips? Is anti-GRP78 BiP Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Based on literature brain does express HSPA5. Based on Uniprot.org, HSPA5 is expressed in vena cava, cervix carcinoma, fibroblast, muscle, articular cartilage, mammary carcinoma, colon carcinoma, brain, cajal-retzius cell fetal brain cortex, melanoma, liver, among other tissues. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-24
Question
We ordered your anti-GRP78 BiP Rabbit Monoclonal antibody for IF on muscle last year. I am using human, and I plan to use the antibody for IHC next. I am interested in examining muscle as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
I took a look at the website and datasheets of our anti-GRP78 BiP Rabbit Monoclonal antibody and I see that M00955 has been validated on human in both IF and IHC. Thus M00955 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-17
Question
My team were well pleased with the WB result of your anti-GRP78 BiP Rabbit Monoclonal antibody. However we have seen positive staining in liver endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is HSPA5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
From what I have seen in literature, liver does express HSPA5. Generally HSPA5 expresses in endoplasmic reticulum lumen. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-01
Question
We are currently using anti-GRP78 BiP Rabbit Monoclonal antibody M00955 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The anti-GRP78 BiP Rabbit Monoclonal antibody (M00955) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-07
