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Product Info Summary
| SKU: | M00955-3 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HSPA5 Antibody
View all GRP78/HSPA5 Antibodies
SKU/Catalog Number
M00955-3
Size
50 µl
Description
Boster Bio Anti-HSPA5 Antibody (Catalog # M00955-3). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HSPA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955-3)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1672CT323.22.82
Isotype
IgG1,k
Immunogen
This HSPA5 antibody is generated from a mouse immunized with a recombinant protein between 420-654 amino acids from human HSPA5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00955-3 is reactive to HSPA5 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
72333 Da
Background of GRP78/HSPA5
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00955-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:20000
Validation Images & Assay Conditions
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All lanes : Anti-HSPA5 Antibody at 1:20000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: mouse liver lysate
Lane 5: rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 72 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For HSPA5 (Source: Uniprot.org, NCBI)
Gene Name
HSPA5
Full Name
Endoplasmic reticulum chaperone BiP
Weight
72333 Da
Superfamily
heat shock protein 70 family
Alternative Names
78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78 HSPA5 BIP, GRP78, HEL-S-89n heat shock protein family A (Hsp70) member 5 endoplasmic reticulum chaperone BiP|78 kDa glucose-regulated protein|HSP70 family protein 5|binding-immunoglobulin protein|endoplasmic reticulum lumenal Ca(2+)-binding protein grp78|epididymis secretory sperm binding protein Li 89n|glucose-regulated protein, 78kDa|heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|heat shock protein 70 family protein 5|heat shock protein family A member 5|immunoglobulin heavy chain-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA5, check out the HSPA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSPA5 Antibody (M00955-3)
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