Product Info Summary
SKU: | PA1760 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-GRO alpha/CXCL1 Antibody Picoband®
View all CXCL1/GRO alpha/KC/CINC-1 Antibodies
SKU/Catalog Number
PA1760
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRO alpha/CXCL1 Antibody catalog # PA1760. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRO alpha/CXCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1760)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GRO alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1760 is reactive to CXCL1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
11301 MW
Background of CXCL1/GRO alpha/KC/CINC-1
CXCL1 (Chemokine, CXC motif, Ligand 1), also called GRO1, SCYB1, GROA or MGSA, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The CXCL1 gene is mapped on 4q13.3. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation,wound healing, and tumorigenesis. Signaling through Cxcr2, Cxcl1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, and concentration dependent, and it reflected enhanced cell/substrate interactions. White matter expression of Cxcl1 was temporospatially regulated. Others contribute to aggressive growth selectivity in the lung. Among the lung metastasis signature genes identified, several, including CXCL1, were functionally validated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1760 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-GRO alpha antibody, PA1760, Western blotting
All lanes: Anti GRO alpha (PA1760) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
Protein Target Info & Infographic
Gene/Protein Information For CXCL1 (Source: Uniprot.org, NCBI)
Gene Name
CXCL1
Full Name
Growth-regulated alpha protein
Weight
11301 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Growth-regulated alpha protein;C-X-C motif chemokine 1;GRO-alpha (1-73);Melanoma growth stimulatory activity;MGSA;Neutrophil-activating protein 3;NAP-3;GRO-alpha (4-73);GRO-alpha (5-73);GRO-alpha (6-73);CXCL1;GRO, GRO1, GROA, MGSA, SCYB1; CXCL1 FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1 C-X-C motif chemokine ligand 1 growth-regulated alpha protein|C-X-C motif chemokine 1|GRO-alpha(1-73)|GRO1 oncogene (melanoma growth stimulating activity, alpha)|GRO1 oncogene (melanoma growth-stimulating activity)|MGSA alpha|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|fibroblast secretory protein|melanoma growth stimulating activity, alpha|melanoma growth stimulatory activity alpha|neutrophil-activating protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL1, check out the CXCL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRO alpha/CXCL1 Antibody Picoband® (PA1760)
Hello CJ!
PA1760 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Crosstalk between NFκB-dependent astrocytic CXCL1 and neuron CXCR2 plays a role in descending pain facilitation Huadong Ni 1, Yungong Wang 2, Kang An 3, Qianying Liu 1, Longsheng Xu 1, Chunyan Zhu 1, Housheng Deng 1, Qiuli He 1, Tingting Wang 1, Miao Xu
Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2
Xu J, Zhu Md, Zhang X, Tian H, Zhang Jh, Wu Xb, Gao Yj. J Neuroinflammation. 2014 Mar 1;11:38. Doi: 10.1186/1742-2094-11-38. Nf??b-Mediated Cxcl1 Production In Spinal Cord Astrocytes Contributes To The Maintenance Of Bone Cancer Pain In Mice.
Chen G, Park Ck, Xie Rg, Berta T, Nedergaard M, Ji Rr. Brain. 2014 Aug;137(Pt 8):2193-209. Doi: 10.1093/Brain/Awu140. Epub 2014 Jun 11. Connexin-43 Induces Chemokine Release From Spinal Cord Astrocytes To Maintain Late-Phase Neuropathic Pain In Mice.
Zhang Zj, Cao Dl, Zhang X, Ji Rr, Gao Yj. Pain. 2013 Oct;154(10):2185-97. Doi: 10.1016/J.Pain.2013.07.002. Epub 2013 Jul 4. Chemokine Contribution To Neuropathic Pain: Respective Induction Of Cxcl1 And Cxcr2 In Spinal Cord Astrocytes And Neurons.
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Customer Q&As
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8 Customer Q&As for Anti-GRO alpha/CXCL1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-GRO alpha/CXCL1 antibody (PA1760)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We do provide the blocking peptide for product anti-GRO alpha/CXCL1 antibody (PA1760). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-12
Question
Will anti-GRO alpha/CXCL1 antibody PA1760 work on feline WB with skin?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Our lab technicians have not validated anti-GRO alpha/CXCL1 antibody PA1760 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GRO alpha/CXCL1 antibody PA1760 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-24
Question
Do you have a BSA free version of anti-GRO alpha/CXCL1 antibody PA1760 available?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GRO alpha/CXCL1 antibody PA1760 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-04
Question
Could I use this antibody to detect mouse samples? Keyword: applications, species sample type, protocol
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
This product can't be used to detect mouse samples.
Boster Scientific Support
Answered: 2018-12-14
Question
I would like to test anti-GRO alpha/CXCL1 antibody PA1760 on human blood for research purposes, then I may be interested in using anti-GRO alpha/CXCL1 antibody PA1760 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Taylor
Verified customer
Asked: 2018-01-09
Answer
The products we sell, including anti-GRO alpha/CXCL1 antibody PA1760, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-09
Question
We are currently using anti-GRO alpha/CXCL1 antibody PA1760 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
The anti-GRO alpha/CXCL1 antibody (PA1760) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
Question
I was wanting to use your anti-GRO alpha/CXCL1 antibody for WB for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
You can see on the product datasheet, PA1760 anti-GRO alpha/CXCL1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-07
Question
I see that the anti-GRO alpha/CXCL1 antibody PA1760 works with WB, what is the protocol used to produce the result images on the product page?
R. Kulkarni
Verified customer
Asked: 2014-04-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-03