Anti-GRB10 Antibody

Product Name

Anti-GRB10 Antibody

View all GRB10 Antibodies

SKU/Catalog Number

A01663

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GRB10 Antibody (Catalog # A01663). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GRB10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GRB10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01663)

Host

Rabbit

Contents

GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10. The immunogen is located within amino acids 440 - 490 of GRB10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.

Reactive Species

A01663 is reactive to GRB10 in Human, Mouse, Rat

Applications

A01663 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

67231 MW

Background of GRB10

GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.

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Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.

Gene/Protein Information For GRB10 (Source: Uniprot.org, NCBI)

Alternative Names

GRB10 adapter protein; GRB10 adaptor protein; Grb-10; GRBIR; GRB-IR; growth factor receptor-bound protein 10; Insulin receptor-binding protein Grb-IR; IRBP; KIAA0207; maternally expressed gene 1; MEG1; RSS GRB10 GRB-IR, Grb-10, IRBP, MEG1, RSS growth factor receptor bound protein 10 growth factor receptor-bound protein 10|GRB10 adapter protein|GRB10 adaptor protein|insulin receptor-binding protein Grb-IR|maternally expressed gene 1

For more info on GRB10, check out the GRB10 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GRB10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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