Click image to see more details
Product Info Summary
| SKU: | EK1261 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse Lumican ELISA Kit PicoKine®
SKU/Catalog Number
EK1261
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Lumican ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lum in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Lumican ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1261)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q19-N338
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1261 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1261 is reactive to Lum in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1261 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lumican
Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1261-CAP | Anti-Mouse Lum Pre-coated 96-well strip microplate | 1 |
| EK1261-ST | Mouse Lum Standard | 2 vials, 10 ng/tube |
| EK1261-DA | Mouse Lum Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.084 | 0.135 | 0.271 | 0.375 | 0.588 | 0.966 | 1.366 | 1.920 |
Data Example Images
Click image to see more details
Mouse Lumican PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lumican, Dilution ratio of 1:5000, concentration in serum and plasma is around 3.2-5 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Lumican ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 240 | 1132 | 5569 | 235 | 1183 | 5831 |
| Standard deviation | 14.88 | 84.9 | 328.57 | 18.8 | 97 | 431.49 |
| CV (%) | 6.2% | 7.5% | 5.9% | 8% | 8.2% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lumican in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 240 | 256 | 285 | 267 | 262 | 16.38 | 6.2% |
| Sample 2 | 1132 | 1232 | 1167 | 1292 | 1205 | 61.37 | 5% |
| Sample 3 | 5569 | 4938 | 5566 | 5489 | 5390 | 263.21 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For Lum (Source: Uniprot.Org, NCBI)
Gene Name
Lum
Full Name
Lumican
Weight
38265 MW
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
Lumican;Keratan sulfate proteoglycan lumican;KSPG lumican;Lum;Lcn, Ldc; LUM LDC, SLRR2D lumican lumican|KSPG lumican|epididymis secretory sperm binding protein|keratan sulfate proteoglycan lumican|lumican proteoglycan
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Lum, check out the Lum Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lum: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Lumican ELISA Kit PicoKine® (EK1261)
Hello CJ!
No publications found for EK1261
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse Lumican ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse Lumican ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Mouse Lumican ELISA Kit PicoKine®
Question
What is the standard that is used for EK1261? What is the protein sequence that is used in the assay? Is there a tag on this protein, if so, what type of tag is used?
Verified customer
Asked: 2022-04-19
Answer
The protein sequence for the standard in the Mouse Lumican ELISA Kit PicoKine® (EK1261) is Q19-N338 (Accession# P51885). A C-terminal 6-His tag is used.
Boster Scientific Support
Answered: 2022-04-20
Question
Would there be a problem with EK1261 to use plasma that was collected with EDTA as an anticoagulant?
Verified customer
Asked: 2020-09-17
Answer
For the Mouse Lumican ELISA Kit PicoKine™ (EK1261), EDTA plasma was not recommended due to the chelating properties. We suggest to collect plasma samples using heparin. If it is not possible, please run a comparison experiment by testing EDTA plasma and heparin plasma to see the effect. However, heparin is recommended.
Boster Scientific Support
Answered: 2020-09-17
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Lumican in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Lumican ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-01-09
Question
Q: is it okay to use citrate plasma as samples in Mouse Lumican Picokine® ELISA Kit (Catalog # EK1261)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Lumican causing disruption of its protein structure. Lumican may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Lumican ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-19
Question
Q: how to proceed with the analysis of ELISA data? I have performed Lumican level in serum.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-09-09
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
S. Robinson
Verified customer
Asked: 2018-11-17
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-11-17
Question
Q: What is the optimal O.D. value for Lumican ELISA kit? I performed your Lumican ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Lumican even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lumican ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lumican that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-11-02
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Lumican using the EK1261 Mouse Lumican Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-23
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-09-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-07-05
