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Product Info Summary
| SKU: | A01958-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-GPCR TGR5/GPBAR1 Antibody Picoband®
View all TGR5/GPBAR1 Antibodies
SKU/Catalog Number
A01958-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GPCR TGR5/GPBAR1 Antibody Picoband® catalog # A01958-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GPCR TGR5/GPBAR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01958-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GPCR TGR5/GPBAR1 recombinant protein (Position: N76-S310).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01958-2 is reactive to GPBAR1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
30570 MW
Background of TGR5/GPBAR1
The G protein-coupled bile acid receptor 1 (GPBAR1) also known G-protein coupled receptor 19 (GPCR19), membrane-type receptor for bile acids (M-BAR) or TGR5 as is a protein that in humans is encoded by the GPBAR1 gene. This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01958-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat PC-12 whole cell, mouse RAW2647 whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GPCR TGR5/GPBAR1 using anti-GPCR TGR5/GPBAR1 antibody (A01958-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR TGR5/GPBAR1 antigen affinity purified polyclonal antibody (Catalog # A01958-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR TGR5/GPBAR1 at approximately 45 kDa. The expected band size for GPCR TGR5/GPBAR1 is at 35 kDa.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-GPCR TGR5/GPBAR1 antibody (A01958-2).
Overlay histogram showing THP-1 cells stained with A01958-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR TGR5/GPBAR1 Antibody (A01958-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GPBAR1 (Source: Uniprot.org, NCBI)
Gene Name
GPBAR1
Full Name
G-protein coupled bile acid receptor 1
Weight
30570 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Insulin-like growth factor-binding protein 5;IBP-5;IGF-binding protein 5;IGFBP-5;IGFBP5;IBP5; GPBAR1 BG37, GPCR19, GPR131, M-BAR, TGR5 G protein-coupled bile acid receptor 1 G-protein coupled bile acid receptor 1|G-protein coupled bile acid receptor BG37|G-protein coupled receptor GPCR19|membrane bile acid receptor|membrane-type receptor for bile acids
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPBAR1, check out the GPBAR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPBAR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPCR TGR5/GPBAR1 Antibody Picoband® (A01958-2)
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