Anti-GP2 (Glycoprotein 2) / ZAP75 Monoclonal Antibody

Product Name

Anti-GP2 (Glycoprotein 2) / ZAP75 Monoclonal Antibody

View all GP2 Antibodies

SKU/Catalog Number

M06630

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-GP2 (Glycoprotein 2) / ZAP75 Monoclonal Antibody (Catalog # M06630). Tested in ELISA, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-GP2 (Glycoprotein 2) / ZAP75 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06630)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: GP2/1712

Isotype

IgG2b, lambda

Immunogen

Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M06630 is reactive to GP2 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

59480 MW

Background of GP2

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06630 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For GP2 (Source: Uniprot.org, NCBI)

Alternative Names

Pancreatic secretory granule membrane major glycoprotein GP2;Pancreatic zymogen granule membrane protein GP-2;ZAP75;GP2; GP2 ZAP75 glycoprotein 2 pancreatic secretory granule membrane major glycoprotein GP2|glycoprotein 2 (zymogen granule membrane)|pancreatic zymogen granule membrane associated protein GP2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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