Anti-GNG2 Antibody (N-Term)

Product Name

Anti-GNG2 Antibody (N-Term)

View all Gng2 Antibodies

SKU/Catalog Number

M06975

Size

50 µl

Description

Boster Bio Anti-GNG2 Antibody (N-Term) (Catalog # M06975). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-GNG2 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06975)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This GNG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from of human GNG2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06975 is reactive to Gng2 in Human, Mouse

Applications

M06975 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

7850 Da

Background of Gng2

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:8000
IHC-P: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-GNG2 Antibody (N-Term) at 1:8000 dilution
Lane 1: human brain lysate
Lane 2: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 8 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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M06975 staining GNG2 in human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For Gng2 (Source: Uniprot.org, NCBI)

Alternative Names

G gamma-I; guanine nucleotide binding protein (G protein), gamma 2; guanine nucleotide binding protein gamma 2; guanine nucleotide-binding protein G(I)/G(O) gamma-2 subunit; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Gng2|guanine nucleotide binding protein (G protein), gamma 2|guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2|g gamma-I

For more info on Gng2, check out the Gng2 Infographic

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In this infographic, you will see the following information for Gng2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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