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Product Info Summary
| SKU: | A09404 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GNAL Antibody
SKU/Catalog Number
A09404
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GNAL Antibody (Catalog # A09404). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GNAL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09404)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GNAL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09404 is reactive to GNAL in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44308 MW
Background of GNAL
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters.
Zigman J.M., Endocrinology 133:2508-2514(1993).
Vuoristo J.T., Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Puhl H.L. III, Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09404 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells, using GNAL antibody A09404.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using GNAL antibody A09404.
Protein Target Info & Infographic
Gene/Protein Information For GNAL (Source: Uniprot.org, NCBI)
Gene Name
GNAL
Full Name
Guanine nucleotide-binding protein G(olf) subunit alpha
Weight
44308 MW
Superfamily
G-alpha family
Alternative Names
Guanine nucleotide-binding protein G (olf) subunit alpha;Adenylate cyclase-stimulating G alpha protein, olfactory type;GNAL; GNAL DYT25 G protein subunit alpha L guanine nucleotide-binding protein G(olf) subunit alpha|adenylate cyclase-stimulating G alpha protein, olfactory type|guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type|guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide, olfactory type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNAL, check out the GNAL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNAL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GNAL Antibody (A09404)
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No publications found for A09404
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GNAL Antibody
Question
I was wanting to use your anti-GNAL antibody for WB for human insulinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human insulinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
You can see on the product datasheet, A09404 anti-GNAL antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human insulinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Will A09404 anti-GNAL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can see on the product datasheet, A09404 anti-GNAL antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-GNAL antibody A09404 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
The anti-GNAL antibody (A09404) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-24
Question
My question regarding product A09404, anti-GNAL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Collins
Verified customer
Asked: 2016-04-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09404 anti-GNAL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-04-04
