Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody

Product Name

Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody

View all Glypican 3 Antibodies

SKU/Catalog Number

M01922

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody (Catalog # M01922). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01922)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: rGPC3/863

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human GPC3 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01922 is reactive to GPC3 in Human, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

65563 MW

Background of Glypican 3

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01922 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For GPC3 (Source: Uniprot.org, NCBI)

Alternative Names

Glypican-3;GTR2-2;Intestinal protein OCI-5;MXR7;Secreted glypican-3;GPC3;OCI5; GPC3 DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1 glypican 3 glypican-3|glypican proteoglycan 3|heparan sulphate proteoglycan|intestinal protein OCI-5|secreted glypican-3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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